Production of anatoxin-a by cyanobacterial strains isolated from Portuguese fresh water systems

The occurrence of anatoxin-a in several freshwater systems in Portugal and its production by Portuguese cyanobacterial strains, after cultivation in laboratory, were studied. Surface water samples from 9 water bodies, for recreational and human consumption usage, were surveyed for anatoxin-a presenc...

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Veröffentlicht in:Ecotoxicology (London, England). - 1992. - 18(2009), 8 vom: 15. Nov., Seite 1110-5
1. Verfasser: Osswald, Joana (VerfasserIn)
Weitere Verfasser: Rellán, Sandra, Gago-Martinez, Ana, Vasconcelos, Vítor
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Ecotoxicology (London, England)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Cyanobacteria Toxins Tropanes Water Pollutants anatoxin a 80023A73NK
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520 |a The occurrence of anatoxin-a in several freshwater systems in Portugal and its production by Portuguese cyanobacterial strains, after cultivation in laboratory, were studied. Surface water samples from 9 water bodies, for recreational and human consumption usage, were surveyed for anatoxin-a presence and for obtaining cultures of pure cyanobacterial strains. Anatoxin-a analysis was performed by high performance liquid chromatography (HPLC) with fluorescence detection (FLD) followed by Mass Spectrometry (MS) confirmation. No anatoxin-a was detected in all the natural water samples (limit of detection (LOD) = 25 ng l(-1)) but among the 22 isolated cyanobacterial strains, 13 could produce anatoxin-a in laboratory conditions (LOD = 3 ng g(-1) dw). This proportion of anatoxin-a producing strains (59.1%) in laboratory is discussed considering the hypothesis that anatoxin-a is a more frequent metabolite in cyanobacteria than it was thought before and making its occurrence in Portuguese freshwaters almost certain. Therefore, health and ecological risks caused by anatoxin-a in Portugal, should be seriously considered 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Cyanobacteria Toxins  |2 NLM 
650 7 |a Tropanes  |2 NLM 
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700 1 |a Rellán, Sandra  |e verfasserin  |4 aut 
700 1 |a Gago-Martinez, Ana  |e verfasserin  |4 aut 
700 1 |a Vasconcelos, Vítor  |e verfasserin  |4 aut 
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