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|a pubmed25n0634.xml
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|a (DE-627)NLM190074841
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|a (NLM)19615133
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Wei, Hong-xia
|e verfasserin
|4 aut
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|a Effects of the treatment with different fluids on alveolar epithelium barrier in rats with acute lung injury
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 11.05.2010
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|a Date Revised 25.11.2016
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To observe the effects of different fluids on alveolar epithelium barrier in rats with acute lung injury (ALI)
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|a METHODS: Thirty-six Sprague-Dawley (SD) rats were randomly assigned into six groups with 6 rats in each group. ALI was induced by intravenous injection of lipopolysaccharide(LPS). Rats in all treatment groups were given different fluids and sacrificed after 4 hours. Evans blue dye (EBD) was injected via the femoral vein 30 minutes before death. Tracheobronchial tree was washed with normal saline (NS) after death, and broncho-alveolar lavage fluid (BALF) was collected. Leakage of EBD from blood into BALF (alveolar epithelial permeability) and wet/dry (W/D) ratio were measured. The mRNA expression of surfactant protein-C (SP-C) was assessed by reverse transcription-polymerase chain reaction (RT-PCR). Alveolar epithelium apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL). Lung injury was evaluated by Smith lung injury score
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|a RESULTS: (1) Lung injury scores in LPS and NS groups were significantly higher than in control group (both P < 0.05). Compared with NS group, lung injury scores were significantly lower in 5% albumin (ALB) and 6% hydroxyethyl starch 130/0.4 (HES) groups (both P < 0.05), and there was no significant difference in 4% succinylated gelatin (GEL) group (P > 0.05). No significant difference was found among the latter three groups. (2) W/D ratio in LPS and NS groups were significantly higher than that in control group (both P < 0.05). Compared with NS group, W/D ratios were lower in ALB, HES and GEL groups (all P < 0.05). But it showed no significant difference among the latter three groups. (3) Alveolar epithelial permeability in LPS and NS groups were remarkably higher than that in control group (both P < 0.05). Compared with NS group, the alveolar epithelial permeability were significantly lower in ALB, HES and GEL groups (all P < 0.05). The alveolar epithelial permeability in the latter two groups were significant lower than that in ALB group (both P < 0.05) but higher than that of control group. (4) The SP-C mRNA expression in LPS, NS, ALB and GEL groups were lower than that in control and HES groups (all P < 0.05), but there was no difference between control and HES groups (P > 0.05). (5) Apoptosis index (AI) of alveolar epithelial cell in all the treatment groups were significantly higher than that in control group (all P < 0.05). Compared with NS group, AI were noticeably lower in ALB and HES groups (both P < 0.05)
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|a CONCLUSION: Compared with NS, colloid can probably improve the alveolar epithelial permeability and protect the barrier function
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|a Comparative Study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Lipopolysaccharides
|2 NLM
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| 700 |
1 |
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|a Yang, Yi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Qiu, Hai-bo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guo, Tao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Ming-ming
|e verfasserin
|4 aut
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| 700 |
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|a Chen, Qiu-hua
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 21(2009), 7 vom: 16. Juli, Seite 412-5
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
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|g volume:21
|g year:2009
|g number:7
|g day:16
|g month:07
|g pages:412-5
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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| 952 |
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|d 21
|j 2009
|e 7
|b 16
|c 07
|h 412-5
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