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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erp187
|2 doi
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|a pubmed24n0631.xml
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|a (NLM)19528530
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wakasa, Yuhya
|e verfasserin
|4 aut
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| 245 |
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|a Expression of unprocessed glutelin precursor alters polymerization without affecting trafficking and accumulation
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 02.12.2009
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|a Date Revised 20.10.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Rice glutelin is synthesized as a precursor in the endosperm endoplasmic reticulum and then deposited within the protein storage vacuole protein body-II (PB-II) as an aggregate, with a high degree of polymerized higher-order structure comprising mature acidic and basic subunits after post-translation processing cleavage. In order to investigate the functional role of this processing and its effect on folding assembly, wild-type GluA2 and its mutant cDNA (mGluA2), in which the conserved processing site (Asn-Gly) at the junction between the acidic and basic chains was replaced with Ala-Ala, were expressed under the control of the endosperm-specific GluB1 promoter in the mutant rice a123 line lacking glutelin GluA1, GluA2, and GluB4. The mGluA2 precursor was synthesized and stably targeted to PB-II without processing in the transgenic rice seeds like the wild-type GluA2. Notably, the saline-soluble mGluA2 precursor assembled with the other type of processed glutelin GluB as a trimer in PB-II, although such hetero-assembly with GluB was not detected in the transformant containing the processed GluA. Furthermore, the mGluA2 precursor in the glutelin fraction was deposited in PB-II by forming a quite different complex from the processed mature GluA2 products. These results indicate that post-translational processing of glutelin is not necessary for trafficking and stable accumulation in PB-II, but is required for the formation of the higher-order structure required for stacking in PB-II
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Protein Precursors
|2 NLM
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| 650 |
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|a Glutens
|2 NLM
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| 650 |
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|a 8002-80-0
|2 NLM
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| 700 |
1 |
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|a Yang, Lijun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hirose, Sakiko
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Takaiwa, Fumio
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 60(2009), 12 vom: 16., Seite 3503-11
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:60
|g year:2009
|g number:12
|g day:16
|g pages:3503-11
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|u http://dx.doi.org/10.1093/jxb/erp187
|3 Volltext
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