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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1080/09593330902753495
|2 doi
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|a pubmed25n0630.xml
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|a (DE-627)NLM189000570
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|a (NLM)19492550
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Vu, Khanh Dang
|e verfasserin
|4 aut
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|a Starch industry wastewater for production of biopesticides--ramifications of solids concentrations
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 19.06.2009
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|a Date Revised 09.12.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a Total solids (TS) concentrations ranging from 15 to 66 g L(-1) of starch industry wastewater (SIW) were tested as raw material for the production of Bacillus thuringiensis var. kurstaki HD-1 (Btk) biopesticide in shake flasks and a 15 L bench-scale fermenter. Shake flask studies revealed a higher delta-endotoxin concentration of Btk at 30 g L(-1) TS concentration and 2.5% (v v(-1)) volume of pre-culture. The fermenter experiments conducted using SIW at 30 g L(-1) TS concentration under controlled conditions of temperature, pH and dissolved oxygen showed higher spore count, enzyme production (protease and amylase) and delta-endotoxin concentration as compared with those of SIW at 15 g L(-1) TS concentration. The entomotoxicity, at the end of fermentation, with SIW at 30 g L(-1) solids concentration (17.8 x 10(9) SBU L(-1), measured against spruce budworm) was considerably higher as compared with entomotoxicity at 15 g L(-1) solids concentration (15.3 x 10(9) SBU L(-1)) and semi-synthetic medium (11.7 x 10(9) SBU L(-1)). The pellet, comprising spores and delta-endotoxin complex obtained after centrifugation and followed by resuspension (in supernatant) in one-tenth of the original volume, of SIW at 30 g L(-1) solids concentration media registered the highest potential for application (to protect forests against spruce budworm) than other media in term of entomotoxicity
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Bacillus thuringiensis Toxins
|2 NLM
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|a Bacterial Proteins
|2 NLM
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|a Endotoxins
|2 NLM
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|a Hemolysin Proteins
|2 NLM
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|a Industrial Waste
|2 NLM
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|a Pesticides
|2 NLM
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|a insecticidal crystal protein, Bacillus Thuringiensis
|2 NLM
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|a Starch
|2 NLM
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|a 9005-25-8
|2 NLM
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|a Tyagi, R D
|e verfasserin
|4 aut
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|a Brar, S K
|e verfasserin
|4 aut
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|a Valéro, J R
|e verfasserin
|4 aut
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|a Surampalli, R Y
|e verfasserin
|4 aut
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|i Enthalten in
|t Environmental technology
|d 1998
|g 30(2009), 4 vom: 01. Apr., Seite 393-405
|w (DE-627)NLM098202545
|x 0959-3330
|7 nnns
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|g volume:30
|g year:2009
|g number:4
|g day:01
|g month:04
|g pages:393-405
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|u http://dx.doi.org/10.1080/09593330902753495
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