Starch industry wastewater for production of biopesticides--ramifications of solids concentrations

Total solids (TS) concentrations ranging from 15 to 66 g L(-1) of starch industry wastewater (SIW) were tested as raw material for the production of Bacillus thuringiensis var. kurstaki HD-1 (Btk) biopesticide in shake flasks and a 15 L bench-scale fermenter. Shake flask studies revealed a higher de...

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Veröffentlicht in:Environmental technology. - 1998. - 30(2009), 4 vom: 01. Apr., Seite 393-405
1. Verfasser: Vu, Khanh Dang (VerfasserIn)
Weitere Verfasser: Tyagi, R D, Brar, S K, Valéro, J R, Surampalli, R Y
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Environmental technology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Bacillus thuringiensis Toxins Bacterial Proteins Endotoxins Hemolysin Proteins Industrial Waste Pesticides insecticidal crystal protein, Bacillus Thuringiensis Starch 9005-25-8
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520 |a Total solids (TS) concentrations ranging from 15 to 66 g L(-1) of starch industry wastewater (SIW) were tested as raw material for the production of Bacillus thuringiensis var. kurstaki HD-1 (Btk) biopesticide in shake flasks and a 15 L bench-scale fermenter. Shake flask studies revealed a higher delta-endotoxin concentration of Btk at 30 g L(-1) TS concentration and 2.5% (v v(-1)) volume of pre-culture. The fermenter experiments conducted using SIW at 30 g L(-1) TS concentration under controlled conditions of temperature, pH and dissolved oxygen showed higher spore count, enzyme production (protease and amylase) and delta-endotoxin concentration as compared with those of SIW at 15 g L(-1) TS concentration. The entomotoxicity, at the end of fermentation, with SIW at 30 g L(-1) solids concentration (17.8 x 10(9) SBU L(-1), measured against spruce budworm) was considerably higher as compared with entomotoxicity at 15 g L(-1) solids concentration (15.3 x 10(9) SBU L(-1)) and semi-synthetic medium (11.7 x 10(9) SBU L(-1)). The pellet, comprising spores and delta-endotoxin complex obtained after centrifugation and followed by resuspension (in supernatant) in one-tenth of the original volume, of SIW at 30 g L(-1) solids concentration media registered the highest potential for application (to protect forests against spruce budworm) than other media in term of entomotoxicity 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Bacillus thuringiensis Toxins  |2 NLM 
650 7 |a Bacterial Proteins  |2 NLM 
650 7 |a Endotoxins  |2 NLM 
650 7 |a Hemolysin Proteins  |2 NLM 
650 7 |a Industrial Waste  |2 NLM 
650 7 |a Pesticides  |2 NLM 
650 7 |a insecticidal crystal protein, Bacillus Thuringiensis  |2 NLM 
650 7 |a Starch  |2 NLM 
650 7 |a 9005-25-8  |2 NLM 
700 1 |a Tyagi, R D  |e verfasserin  |4 aut 
700 1 |a Brar, S K  |e verfasserin  |4 aut 
700 1 |a Valéro, J R  |e verfasserin  |4 aut 
700 1 |a Surampalli, R Y  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Environmental technology  |d 1998  |g 30(2009), 4 vom: 01. Apr., Seite 393-405  |w (DE-627)NLM098202545  |x 0959-3330  |7 nnns 
773 1 8 |g volume:30  |g year:2009  |g number:4  |g day:01  |g month:04  |g pages:393-405 
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