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231223s2008 xx ||||| 00| ||eng c |
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|a pubmed25n0629.xml
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|a (NLM)19461940
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Handa, Hitesh
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Recognition of Salmonella Typhimurium by Immobilized Phage P22 Monolayers
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| 264 |
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1 |
|c 2008
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Revised 12.03.2024
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a Phages are promising alternatives to antibodies as the biorecognition element in a variety of biosensing applications. In this study, a monolayer of bacteriophage P22 whose tailspike proteins specifically recognize Salmonella serotypes was covalently bound to glass substrates through a bifunctional cross linker 3-aminopropyltrimethoxysilane. The specific binding of Salmonella typhimurium to the phage monolayer was studied by enzyme-linked immunosorbent assay and atomic force microscopy. Escherichia coli and a Gram-positive bacterium Listeria monocytogenes were also studied as control bacteria. The P22 particles show strong binding affinity to Salmonella typhimurium. In addition, the dried P22 monolayer maintained 50% binding capacity to Salmonella typhimurium after a one-week storage time. This is a promising method to prepare phage monolayer coatings on surface plasmon resonance and acoustic biosensor substrates in order to utilize the nascent phage display technology
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| 650 |
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|a Journal Article
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| 700 |
1 |
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|a Gurczynski, Stephen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jackson, Matthew P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Auner, Gregory
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mao, Guangzhao
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Surface science
|d 1997
|g 602(2008), 7 vom: 01. Apr., Seite 1392-1400
|w (DE-627)NLM098126490
|x 0039-6028
|7 nnns
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| 773 |
1 |
8 |
|g volume:602
|g year:2008
|g number:7
|g day:01
|g month:04
|g pages:1392-1400
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 602
|j 2008
|e 7
|b 01
|c 04
|h 1392-1400
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