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231223s2009 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1021/la803205n
|2 doi
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|a eng
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| 100 |
1 |
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|a Kim, Kwan
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Metal-enhanced fluorescence of rhodamine B isothiocyanate from micrometer-sized silver powders
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| 264 |
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1 |
|c 2009
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 01.07.2009
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|a Date Revised 13.05.2009
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a We report here the use of micrometer-sized silver (microAg) powders to enhance the fluorescence emission of rhodamine B isothiocyanate (RhBITC). Our findings clearly show that the RhBITC displays up to a 36-fold increase in fluorescence emission intensity when RhBITC-labeled poly(allylamine hydrochloride) (RhBITC-PAH) is assembled onto a polyelectrolyte layer (about 30 nm thick) above microAg, when compared to that measured for RhBITC-PAH assembled directly onto a glass slide. A similar experiment conducted using a vacuum-evaporated Ag film displayed only a 5-fold increase. The enhanced fluorescence observed for microAg powders is obviously due to their ability to concentrate the electric fields. This suggests that metal-enhanced fluorescence (MEF) can potentially be employed to increase the sensitivity and detection limit of various bioassays that employ RhBITC as a fluorescence label. Since microAg powders are also an efficient substrate for surface-enhanced Raman scattering (SERS), molecular sensors operating via both SERS and MEF may then be able to be fabricated using microAg powders
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|a Journal Article
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| 700 |
1 |
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|a Lee, Yoon Mi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Ji Won
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shin, Kuan Soo
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1991
|g 25(2009), 5 vom: 03. März, Seite 2641-5
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|x 1520-5827
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|g volume:25
|g year:2009
|g number:5
|g day:03
|g month:03
|g pages:2641-5
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|u http://dx.doi.org/10.1021/la803205n
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