Characterization of a sabinene synthase gene from rough lemon (Citrus jambhiri)

Characterization of a sabinene synthase gene from rough lemon (Citrus jambhiri)

We previously isolated two putative monoterpene synthase genes, RlemTPS1 and RlemTPS2, from rough lemon (Citrus jambhiri) and showed that gene expression of RlemTPS2 was induced by microbial attack. The protein product of RlemTPS2 was obtained using a prokaryotic expression system, and GC and GC-MS...

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Veröffentlicht in:Journal of plant physiology. - 1979. - 166(2009), 15 vom: 15. Okt., Seite 1700-4
1. Verfasser: Kohzaki, Keisuke (VerfasserIn)
Weitere Verfasser: Gomi, Kenji, Yamasaki-Kokudo, Yumiko, Ozawa, Rika, Takabayashi, Junji, Akimitsu, Kazuya
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Antifungal Agents Bicyclic Monoterpenes Monoterpenes Plant Proteins sabinene 7D1TL44GPC Intramolecular Lyases EC 5.5.- pinene cyclase I
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520 |a We previously isolated two putative monoterpene synthase genes, RlemTPS1 and RlemTPS2, from rough lemon (Citrus jambhiri) and showed that gene expression of RlemTPS2 was induced by microbial attack. The protein product of RlemTPS2 was obtained using a prokaryotic expression system, and GC and GC-MS of monoterpene synthesis by RlemTPS2 determined that RlemTPS2 encodes a sabinene synthase. Sabinene has antifungal activity toward Alternaria alternata. Furthermore, site-directed mutagenesis identified one amino acid, Ile, located at the front of the metal ion binding motif as an important residue for the product specificity of sabinene synthase 
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700 1 |a Yamasaki-Kokudo, Yumiko  |e verfasserin  |4 aut 
700 1 |a Ozawa, Rika  |e verfasserin  |4 aut 
700 1 |a Takabayashi, Junji  |e verfasserin  |4 aut 
700 1 |a Akimitsu, Kazuya  |e verfasserin  |4 aut 
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