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20231223181710.0 |
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231223s2009 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1021/la900545t
|2 doi
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|a pubmed24n0628.xml
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|a (DE-627)NLM188308385
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|a (NLM)19419188
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Maus, Lisa
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Quantification and reactivity of functional groups in the ligand shell of PEGylated gold nanoparticles via a fluorescence-based assay
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| 264 |
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 17.09.2009
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|a Date Revised 01.12.2018
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|a published: Print
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|a Citation Status MEDLINE
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|a We present a fluorescence-based assay for the characterization of functionalized gold nanoparticles (AuNPs) capped with a self-assembled monolayer of mixed thiols derived from poly(ethylene glycol) (PEG). These water-soluble AuNPs carry primary amino groups at the solvent-exposed interface, which can be used for further conjugation of biologically active molecules. The reported assay allows quantification of the average number of functionalizable amino groups per particle (N(NH(2))) with a relative uncertainty below or equal to +/-14% (95% confidence interval), thus providing essential information for the successive derivatization of the AuNPs. Here, a fluorescently labeled derivative of peptide-neurotoxin conantokin-G was coupled to the amino groups of the particle ligand shell via a flexible linker. We quantitatively determined the average number of peptides per particle (N(pept)) and the yield of the two-step conjugation strategy. AuNPs carrying 50-70 copies of the peptide were obtained. In addition, we have gained insights into the deterioration of the self-assembled monolayer due to thiol desorption with time. Under ordinary storage conditions in solution and at room temperature, a decrease in N(NH(2)) between 48% and 75% could be observed at the end of the period of investigation (42-56 days). Slow desorption of the conjugated peptides upon storage was also observed and quantified ( approximately 25% in 14 days)
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4 |
|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Polyethylene Glycols
|2 NLM
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| 650 |
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7 |
|a 3WJQ0SDW1A
|2 NLM
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| 650 |
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|a Gold
|2 NLM
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| 650 |
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7 |
|a 7440-57-5
|2 NLM
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| 700 |
1 |
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|a Spatz, Joachim P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fiammengo, Roberto
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 25(2009), 14 vom: 21. Juli, Seite 7910-7
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:25
|g year:2009
|g number:14
|g day:21
|g month:07
|g pages:7910-7
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| 856 |
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|u http://dx.doi.org/10.1021/la900545t
|3 Volltext
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