Patterning of HeLa cells on a microfabricated Au-coated ITO substrate
HeLa cells were patterned on a microfabricated Au-coated ITO substrate. Part of the Au film was dissolved by the electrochemical wet stamping technique, and the substrate was then immersed in a thiol-terminated methoxy-poly(ethylene glycol) (mPEG-SH) solution for further modification. Self-assembled...
| Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1991. - 25(2009), 9 vom: 05. Mai, Seite 5380-3 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2009
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| Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Tin Compounds Indium 045A6V3VFX Gold 7440-57-5 stannic oxide KM7N50LOS6 |
| Résumé: | HeLa cells were patterned on a microfabricated Au-coated ITO substrate. Part of the Au film was dissolved by the electrochemical wet stamping technique, and the substrate was then immersed in a thiol-terminated methoxy-poly(ethylene glycol) (mPEG-SH) solution for further modification. Self-assembled monolayers (SAMs) on the gold island can form cell- and protein-resistant regions on the substrate, and living HeLa cells can adhere to the exposed ITO surface. Single cell patterns could be achieved when the size of a microstructure unit matched that of the cell. The fluorescence experiments further proved that living HeLa cells prefer to attach to the ITO substrate rather than the mPEG-SH-modified gold islands |
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| Description: | Date Completed 02.06.2009 Date Revised 25.11.2016 published: Print Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la804297x |