Root development under metal stress in Arabidopsis thaliana requires the H+/cation antiporter CAX4
* The Arabidopsis vacuolar CAtion eXchangers (CAXs) play a key role in mediating cation influx into the vacuole. In Arabidopsis, there are six CAX genes. However, some members are yet to be characterized fully. * In this study, we show that CAX4 is expressed in the root apex and lateral root primord...
| Publié dans: | The New phytologist. - 1979. - 183(2009), 1 vom: 12., Seite 95-105 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2009
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| Accès à la collection: | The New phytologist |
| Sujets: | Journal Article Research Support, U.S. Gov't, Non-P.H.S. Antiporters Arabidopsis Proteins CAX4 protein, Arabidopsis Cation Transport Proteins Indoleacetic Acids Metals, Heavy |
| Résumé: | * The Arabidopsis vacuolar CAtion eXchangers (CAXs) play a key role in mediating cation influx into the vacuole. In Arabidopsis, there are six CAX genes. However, some members are yet to be characterized fully. * In this study, we show that CAX4 is expressed in the root apex and lateral root primordia, and that expression is increased when Ni(2+) or Mn(2+) levels are elevated or Ca(2+) is depleted. * Transgenic plants expressing increased levels of CAX4 display symptoms consistent with increased sequestration of Ca(2+) and Cd(2+) into the vacuole. When CAX4 is highly expressed in an Arabidopsis cax1 mutant line with weak vacuolar Ca(2+)/H(+) antiport activity, a 29% increase in Ca(2+)/H(+) antiport is measured. A cax4 loss-of-function mutant and CAX4 RNA interference lines display altered root growth in response to Cd(2+), Mn(2+) and auxin. The DR5::GUS auxin reporter detected reduces auxin responses in the cax4 lines. * These results indicate that CAX4 is a cation/H(+) antiporter that plays an important function in root growth under heavy metal stress conditions |
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| Description: | Date Completed 30.11.2009 Date Revised 08.04.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/j.1469-8137.2009.02831.x |