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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1021/la802075t
|2 doi
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|a pubmed24n0626.xml
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|a (DE-627)NLM187764239
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|a (NLM)19360936
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Jeanquartier, Claire
|e verfasserin
|4 aut
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|a A two-step method to covalently bind biomolecules to group-IV semiconductors
|b Si(111)/1,2-epoxy-9-decene/esterase
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 01.05.2009
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a A versatile two-step method has been developed that allows linking of biomolecules covalently to hydrogen-terminated group-IV semiconductors by means of epoxy-alkenes. First, the terminal C==C double bond of the alkene forms a covalent bond with the silicon, germanium, or diamond surface by UV-mediated hydrosilylation. The terminal oxirane moiety then reacts with the biomolecule. As a model system, we investigated the attachment of an esterase B to a Si(111) surface by means of the linker molecule 1,2-epoxy-9-decene. Samples were characterized by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy. The immobilized enzyme retained its activity and exhibited good long-term stability
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Epoxy Compounds
|2 NLM
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|a Esterases
|2 NLM
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|a EC 3.1.-
|2 NLM
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|a Silicon
|2 NLM
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|a Z4152N8IUI
|2 NLM
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1 |
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|a Schider, Gerburg
|e verfasserin
|4 aut
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|a Feichtenhofer, Sabine
|e verfasserin
|4 aut
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1 |
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|a Schwab, Helmut
|e verfasserin
|4 aut
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|a Schennach, Robert
|e verfasserin
|4 aut
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|a Stettner, Johanna
|e verfasserin
|4 aut
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1 |
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|a Winkler, Adolf
|e verfasserin
|4 aut
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|a Gruber-Woelfler, Heidrun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schitter, Georg
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Eder, Rafael J P
|e verfasserin
|4 aut
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| 700 |
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|a Khinast, Johannes G
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 24(2008), 24 vom: 16. Dez., Seite 13957-61
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:24
|g year:2008
|g number:24
|g day:16
|g month:12
|g pages:13957-61
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|u http://dx.doi.org/10.1021/la802075t
|3 Volltext
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|h 13957-61
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