Effect of the ferredoxin electron donor on sunflower (Helianthus annuus) desaturases

Ferredoxins are proteins that participate in photosynthesis and in other processes that require reducing equivalents, such as the reduction of nitrogen or fatty acid desaturation. Two classes of ferredoxins have been described in plants: light-regulated photosynthetic ferredoxins and heterotrophic f...

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Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 47(2009), 8 vom: 15. Aug., Seite 657-62
1. Verfasser: Venegas-Calerón, M (VerfasserIn)
Weitere Verfasser: Youssar, L, Salas, J J, Garcés, R, Martínez-Force, E
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Ferredoxins Reducing Agents alpha-Linolenic Acid 0RBV727H71 Cytochromes c 9007-43-6 Linoleic Acid 9KJL21T0QJ mehr... Mixed Function Oxygenases EC 1.- Fatty Acid Desaturases EC 1.14.19.- acyl-(acyl-carrier-protein)desaturase EC 1.14.19.2
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245 1 0 |a Effect of the ferredoxin electron donor on sunflower (Helianthus annuus) desaturases 
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520 |a Ferredoxins are proteins that participate in photosynthesis and in other processes that require reducing equivalents, such as the reduction of nitrogen or fatty acid desaturation. Two classes of ferredoxins have been described in plants: light-regulated photosynthetic ferredoxins and heterotrophic ferredoxins whose activity is not influenced by light. Genes encoding the two forms of ferredoxin have been cloned and characterized in developing sunflower cotyledons. Here, these genes were overexpressed in Escherichia coli and they were purified by ion exchange and size exclusion chromatography to study their capacity to supply electrons to two different sunflower desaturases: soluble stearoyl-ACP desaturase from sunflower cotyledons, and membrane bound desaturase FAD7 expressed in yeast. In both cases photosynthetic ferredoxin was the form that promoted the strongest desaturase activity 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Ferredoxins  |2 NLM 
650 7 |a Reducing Agents  |2 NLM 
650 7 |a alpha-Linolenic Acid  |2 NLM 
650 7 |a 0RBV727H71  |2 NLM 
650 7 |a Cytochromes c  |2 NLM 
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650 7 |a Linoleic Acid  |2 NLM 
650 7 |a 9KJL21T0QJ  |2 NLM 
650 7 |a Mixed Function Oxygenases  |2 NLM 
650 7 |a EC 1.-  |2 NLM 
650 7 |a Fatty Acid Desaturases  |2 NLM 
650 7 |a EC 1.14.19.-  |2 NLM 
650 7 |a acyl-(acyl-carrier-protein)desaturase  |2 NLM 
650 7 |a EC 1.14.19.2  |2 NLM 
700 1 |a Youssar, L  |e verfasserin  |4 aut 
700 1 |a Salas, J J  |e verfasserin  |4 aut 
700 1 |a Garcés, R  |e verfasserin  |4 aut 
700 1 |a Martínez-Force, E  |e verfasserin  |4 aut 
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773 1 8 |g volume:47  |g year:2009  |g number:8  |g day:15  |g month:08  |g pages:657-62 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2009.03.005  |3 Volltext 
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