Nanomechanical properties of dead or alive single-patterned bacteria

The main goal of this paper is to probe mechanical properties of living and dead bacteria via atomic force microscopy (AFM) indentation experimentations. Nevertheless, the prerequisite for bioAFM study is the adhesion of the biological sample on a surface. Although AFM has now been used in microbiol...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 25(2009), 10 vom: 19. Mai, Seite 5731-6
1. Verfasser: Cerf, Aline (VerfasserIn)
Weitere Verfasser: Cau, Jean-Christophe, Vieu, Christophe, Dague, Etienne
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't
Beschreibung
Zusammenfassung:The main goal of this paper is to probe mechanical properties of living and dead bacteria via atomic force microscopy (AFM) indentation experimentations. Nevertheless, the prerequisite for bioAFM study is the adhesion of the biological sample on a surface. Although AFM has now been used in microbiology for 20 years, the immobilization of micro-organisms is still challenging. Immobilizing a single cell, without the need for chemical fixation has therefore constituted our second purpose. Highly ordered arrays of single living bacteria were generated over the millimeter scale by selective adsorption of bacteria onto micrometric chemical patterns. The chemically engineered template surfaces were prepared with a microcontact printing process, and different functionalizations of the patterns by incubation were investigated. Thanks to this original immobilization strategy, the Young moduli of the same cell were measured using force spectroscopy before and after heating (45 degrees C, 20 min). The cells with a damaged membrane (after heating) present a Young modulus twice as high as that of healthy bacteria
Beschreibung:Date Completed 23.07.2009
Date Revised 12.05.2009
published: Print
Citation Status MEDLINE
ISSN:1520-5827
DOI:10.1021/la9004642