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01000caa a22002652c 4500 |
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NLM187421978 |
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DE-627 |
| 005 |
20250210071756.0 |
| 007 |
tu |
| 008 |
231223s1970 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed25n0625.xml
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| 035 |
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|a (DE-627)NLM187421978
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| 035 |
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|a (NLM)19322277
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| 040 |
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Buecher, E J
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A Nematode Growth Factor from Baker's Yeast
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| 264 |
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1 |
|c 1970
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| 336 |
|
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 14.07.2011
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| 500 |
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|a Date Revised 20.10.2021
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| 500 |
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|a published: Print
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| 500 |
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|a ErratumIn: J Nematol. 1971 Jan;3(1):100.. - PMID 19322353
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| 500 |
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a An extract prepared from commercially available yeast supported maturation of the free-living nematode Caenorhabditis briggsae. The extract can be used to supplement a chemically defined medium or, after a limited dialysis, as a complete medium. Several biologically active fractions were prepared; those containing larger amounts of ribonucleic acid (RNA) had greater biological activity, the most active being a pellet resuspended after centrifugation at 30,000 x g for 30 min. This fraction could be substituted for serum in a medium which supports the maturation of the animal parasites Trichinella spiralis and Hymenolepis nana. Addition of protamine sulfate decreased the RNA content, leaving inactive protein fractions which could be reactivated by specific treatments that caused protein precipitation. It is postulated that biological activity is associated with protein sedimented with ribosomes
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Axenic culture
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| 650 |
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4 |
|a Caenorhabditis briggsae
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| 650 |
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4 |
|a Particle formation
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| 650 |
|
4 |
|a Ribosomes
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| 700 |
1 |
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|a Hansen, E L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gottfried, T
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of nematology
|d 1969
|g 2(1970), 1 vom: 13. Jan., Seite 93-8
|w (DE-627)NLM098196421
|x 0022-300X
|7 nnas
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| 773 |
1 |
8 |
|g volume:2
|g year:1970
|g number:1
|g day:13
|g month:01
|g pages:93-8
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 2
|j 1970
|e 1
|b 13
|c 01
|h 93-8
|