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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2009.02.012
|2 doi
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|a pubmed24n0625.xml
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|a (DE-627)NLM187413312
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|a (NLM)19321357
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Casani, Simone
|e verfasserin
|4 aut
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| 245 |
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|a Investigation on cell death in the megagametophyte of Araucaria bidwillii Hook. post-germinated seeds
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 13.08.2009
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The megagametophyte of the Araucaria bidwillii seed is a storage tissue that surrounds and feeds the embryo. When all its reserves are mobilized, the megagametophyte degenerates as a no longer needed tissue. In this work we present a biochemical and a cytological characterization of the megagametophyte cell death. The TUNEL assay showed progressive DNA fragmentation throughout the post-germinative stages, while DNA electrophoretic analysis highlighted a smear as the predominant pattern of DNA degradation and internucleosomal DNA cleavage only for a minority of cells at late post-germinative stages. Cytological investigations at these stages detected profound changes in the size and morphology of the megagametophyte nuclei. By using in vitro assays, we were able to show a substantial increase in proteolytic activities, including caspase-like protease activities during the megagametophyte degeneration. Among the caspase-like enzymes, caspase 6- and 1-like proteases appeared to be the most active in the megagametophyte with a preference for acidic pH. On the basis of our results, we propose that the major pathway of cell death in the Araucaria bidwillii megagametophyte is necrosis; however, we do not exclude that some cells undergo developmental programmed cell death
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|a Journal Article
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|a DNA, Plant
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Peptide Hydrolases
|2 NLM
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|a EC 3.4.-
|2 NLM
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|a Caspases
|2 NLM
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|a EC 3.4.22.-
|2 NLM
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| 700 |
1 |
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|a Fontanini, Debora
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Capocchi, Antonella
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lombardi, Lara
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Galleschi, Luciano
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 47(2009), 7 vom: 15. Juli, Seite 599-607
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:47
|g year:2009
|g number:7
|g day:15
|g month:07
|g pages:599-607
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|u http://dx.doi.org/10.1016/j.plaphy.2009.02.012
|3 Volltext
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