Zn stress-induced inhibition of bean PvSR2-GUS fusion gene splicing is gene-specific in transgenic tobacco
The stress-related gene no. 2 of Phaseolus vulgaris (PvSR2) is metal inducible and contains a single intron. Here, we report that Zn stress inhibited the splicing of the PvSR2-beta-glucuronidase (GUS) fusion gene in a concentration- and time-dependent manner in tobacco seedlings. The inhibition appe...
| Veröffentlicht in: | Journal of plant physiology. - 1979. - 166(2009), 11 vom: 15. Juli, Seite 1223-7 |
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| Weitere Verfasser: | , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2009
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| Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Zinc J41CSQ7QDS |
| Zusammenfassung: | The stress-related gene no. 2 of Phaseolus vulgaris (PvSR2) is metal inducible and contains a single intron. Here, we report that Zn stress inhibited the splicing of the PvSR2-beta-glucuronidase (GUS) fusion gene in a concentration- and time-dependent manner in tobacco seedlings. The inhibition appears to be specific for the PvSR2-GUS transgene: splicing of four endogenous tobacco genes was unaffected by Zn stress. Our results provide in vivo evidence that Zn stress-dependent intron retention is transgene specific in plants |
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| Beschreibung: | Date Completed 02.09.2009 Date Revised 13.12.2023 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1618-1328 |
| DOI: | 10.1016/j.jplph.2009.01.010 |