Isolation of Subcellular Granules from Second-Stage Juveniles of Meloidogyne incognita

Subcellular granules from the second-stage (preparasitic) juveniles of root-knot nematode Meloidogyne incognita were isolated by isopycnic centrifugation on Percoll. The granules had an apparent density of 1.13 g/cm(3). The relative specific activity of acid phosphatase in the granule extract was 8....

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Veröffentlicht in:Journal of nematology. - 1969. - 17(1985), 4 vom: 28. Okt., Seite 482-8
1. Verfasser: Reddigari, S R (VerfasserIn)
Weitere Verfasser: Sundermann, C A, Hussey, R S
Format: Aufsatz
Sprache:English
Veröffentlicht: 1985
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article acid phosphatase esophageal gland giant cell root-knot nematode secretory granule
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520 |a Subcellular granules from the second-stage (preparasitic) juveniles of root-knot nematode Meloidogyne incognita were isolated by isopycnic centrifugation on Percoll. The granules had an apparent density of 1.13 g/cm(3). The relative specific activity of acid phosphatase in the granule extract was 8.4. Acid phosphatase activity was also detected histochemically in the subventral gland granules. Electron microscopy and malate dehydrogenase activity indicated that contamination of granules by mitochondria was negligible. Electrophoresis of the granule extract in the presence of sodium dodecyl sulfate showed 15-20 major protein bands 
650 4 |a Journal Article 
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700 1 |a Hussey, R S  |e verfasserin  |4 aut 
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