Comparative Electrophoretic Analyses of Soluble Proteins from Heterodera glycines Races 1-4 and Three Other Heterodera Species

Modified polyacrylamide gel and SDS-polyacrylamide gel electrophoretic systems using a low molarity tris-HCl buffer and equal pH of homogenizing buffer and stacking gel provided improved stacking for separation of soluble proteins from Heterodera schachtii, H. trifolii, H. lespedezae, and H. glycine...

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Veröffentlicht in:Journal of nematology. - 1969. - 16(1984), 3 vom: 28. Juli, Seite 332-40
1. Verfasser: Pozdol, R F (VerfasserIn)
Weitere Verfasser: Noel, G R
Format: Aufsatz
Sprache:English
Veröffentlicht: 1984
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Heterodera lespedezae Heterodera schachtii Heterodera trifolii electrophoresis soybean cyst nematode
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245 1 0 |a Comparative Electrophoretic Analyses of Soluble Proteins from Heterodera glycines Races 1-4 and Three Other Heterodera Species 
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520 |a Modified polyacrylamide gel and SDS-polyacrylamide gel electrophoretic systems using a low molarity tris-HCl buffer and equal pH of homogenizing buffer and stacking gel provided improved stacking for separation of soluble proteins from Heterodera schachtii, H. trifolii, H. lespedezae, and H. glycines races 1, 2, 3, and 4, compared with previous studies with cyst nematodes, The four Heterodera species were easily distinguished using the polyacrylamide gel system, but H. trifolii and H. lespedezae had similar protein patterns. H. glycines races were not separable by that system. The SDS-polyacrylamide gel system produced different protein patterns for all four Heterodera species although H. trifolii and H. lespedezae differed by only a single band, suggesting that these two may be subspecifically related. A protein band unique to H. glycines races 3 and 4 was not detected in SDS-polyacrylamide gel profiles from races 1 and 2. Molecular weight determinations were 55,000 for distinctive proteins in profiles of H. trifolii and 75,000 for H. glycines races 3 and 4 
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650 4 |a Heterodera lespedezae 
650 4 |a Heterodera schachtii 
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650 4 |a electrophoresis 
650 4 |a soybean cyst nematode 
700 1 |a Noel, G R  |e verfasserin  |4 aut 
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