Genomic Differences among Pathotypes of Bursaphelenchus xylophilus

Total genomic DNA from Bursaphelenchus xylophilus pathotypes MPSy-1 and VPSt-1 and from B. mucronatus was digested with restriction endonucleases. DNA fragments were electrophoretically separated, Southern blotted to nitrocellulose, and hybridized to genomic DNA from one of the isolates. The resulti...

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Détails bibliographiques
Publié dans:Journal of nematology. - 1969. - 20(1988), 2 vom: 15. Apr., Seite 309-16
Auteur principal: Bolla, R I (Auteur)
Autres auteurs: Weaver, C, Winter, R E
Format: Article
Langue:English
Publié: 1988
Accès à la collection:Journal of nematology
Sujets:Journal Article Abies balsamea B. xylophilus Bursaphelenchus mucronatus DNA P. strobus P. sylvestris Pinus nigra genetics pathotype plus... pinewood nematode restriction endonuclease
Description
Résumé:Total genomic DNA from Bursaphelenchus xylophilus pathotypes MPSy-1 and VPSt-1 and from B. mucronatus was digested with restriction endonucleases. DNA fragments were electrophoretically separated, Southern blotted to nitrocellulose, and hybridized to genomic DNA from one of the isolates. The resulting hybridization patterns indicate genomic differences in repetitive DNA sequences among these populations. Greatest differences were seen between B. xylophilus and B. mucronatus, but genomic differences were also apparent between B. xylophilus pathotypes MPSy-1 and VPSt-1 and between a population from P. nigra in New Jersey and a population of a mucronate form from Abies balsamea in Quebec, Canada
Description:Date Completed 14.07.2011
Date Revised 20.10.2021
published: Print
Citation Status PubMed-not-MEDLINE
ISSN:0022-300X