Efficient Procedure for Extracting Tylenchulus semipenetrans from Citrus Roots
Investigations were undertaken to determine the suitability of sucrose and magnesium sulphate solutions and a silica colloidal suspension with centrifugation for extracting Tylenchulus semipenetrans from citrus roots. The efficiency of incubation, sodium hypochlorite, centrifugation, and maceration...
| Publié dans: | Journal of nematology. - 1969. - 22(1990), 4 vom: 16. Okt., Seite 590-3 |
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| Format: | Article |
| Langue: | English |
| Publié: |
1990
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| Accès à la collection: | Journal of nematology |
| Sujets: | Journal Article Citrus aurantium Tylenchulus semipenetrans citrus nematode extraction method sour orange |
| Résumé: | Investigations were undertaken to determine the suitability of sucrose and magnesium sulphate solutions and a silica colloidal suspension with centrifugation for extracting Tylenchulus semipenetrans from citrus roots. The efficiency of incubation, sodium hypochlorite, centrifugation, and maceration methods was also compared. Numbers of females recovered by centrifugation with colloidal silica were greater than those from sucrose or magnesium sulphate. Incubation, sodium hypochlorite, and centrifugation methods were satisfactory for extracting eggs, second-stage juveniles, and males, whereas the maceration-sieving method was less efficient. Combining the sodium hypochlorite method with a 15-second maceration followed by centrifugation in colloidal silica reduced the recovery of T. semipenetrans females from citrus roots |
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| Description: | Date Completed 14.07.2011 Date Revised 20.10.2021 published: Print Citation Status PubMed-not-MEDLINE |
| ISSN: | 0022-300X |