|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM187096155 |
| 003 |
DE-627 |
| 005 |
20250210063033.0 |
| 007 |
tu |
| 008 |
231223s1990 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed25n0624.xml
|
| 035 |
|
|
|a (DE-627)NLM187096155
|
| 035 |
|
|
|a (NLM)19287752
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Harris, T S
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Identification of single meloidogyne juveniles by polymerase chain reaction amplification of mitochondrial DNA
|
| 264 |
|
1 |
|c 1990
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 14.07.2011
|
| 500 |
|
|
|a Date Revised 20.10.2021
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status PubMed-not-MEDLINE
|
| 520 |
|
|
|a Polymerase chain reaction (PCR) was used to amplify a specific 1.8-kb sequence of mitochondrial DNA from single juveniles and eggs from 17 populations of Meloidogyne incognita, M. hapla, M. javanica, and M. arenaria. Approximately 2 mug amplified product were produced per reaction. Restriction digestion of the amplified product with HinfI permitted discrimination of clonal lineages of the four species. Meloidogyne javanica, however, could not be separated from M. hapla by the enzymes used in these experiments. Various amplification conditions and nematode lysis procedures were examined in order to optimize the speed and quality of identifications
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Meloidogyne spp.
|
| 650 |
|
4 |
|a diagnostics
|
| 650 |
|
4 |
|a mitochondrial DNA
|
| 650 |
|
4 |
|a polymerase chain reaction (PCR)
|
| 650 |
|
4 |
|a root-knot nematode
|
| 700 |
1 |
|
|a Sandall, L J
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Powers, T O
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of nematology
|d 1969
|g 22(1990), 4 vom: 16. Okt., Seite 518-24
|w (DE-627)NLM098196421
|x 0022-300X
|7 nnns
|
| 773 |
1 |
8 |
|g volume:22
|g year:1990
|g number:4
|g day:16
|g month:10
|g pages:518-24
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 22
|j 1990
|e 4
|b 16
|c 10
|h 518-24
|