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01000caa a22002652c 4500 |
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NLM187054533 |
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DE-627 |
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20250210062432.0 |
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tu |
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231223s1992 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed25n0624.xml
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| 035 |
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|a (DE-627)NLM187054533
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| 035 |
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|a (NLM)19283206
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| 040 |
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Halbrendt, J M
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A Technique for Evaluating Heterodera glycines Development in Susceptible and Resistant Soybean
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| 264 |
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1 |
|c 1992
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 14.07.2011
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| 500 |
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|a Date Revised 20.10.2021
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a A technique was developed to evaluate Heterodera glycines development in susceptible and resistant soybean. Roots of 3-day-old soybean were exposed to infective juveniles of H. glyci.nes in sand for 8 hours followed by washing and transfer to hydroponic culture. The cotyledons and apical meristem were removed and plants were maintained under constant light, which resulted in a dwarfed plant system. After 15 or 20 days at 27 C, nematodes were rated for development. Emerged males were sieved from the culture water and females were counted directly from the roots. Nematodes remaining in the roots were rated for development after staining and clearing the tissues. The proportion of nematodes at each stage of development and the frequency of completed molts for each stage were calculated from these data. This technique showed that resistance to H. glycines was stage related and did not affect males and females equally in all resistant hosts. The resistance of plant introduction PI 209332 primarily affected development of third and fourth-stage juveniles; 'Pickett' mainly affected second and third-stage juveniles, whereas PI 89772 affected all stages. Male development was markedly affected in PI 89772 and 'Pickett' but not in PI 209332
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Glycine max
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| 650 |
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4 |
|a Heterodera glycines
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| 650 |
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4 |
|a cyst nematode
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| 650 |
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4 |
|a hydroponics
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| 650 |
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4 |
|a nematode
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| 650 |
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4 |
|a resistance
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| 650 |
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4 |
|a soybean
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| 700 |
1 |
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|a Lewis, S A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shipe, E R
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of nematology
|d 1969
|g 24(1992), 1 vom: 15. März, Seite 84-91
|w (DE-627)NLM098196421
|x 0022-300X
|7 nnas
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| 773 |
1 |
8 |
|g volume:24
|g year:1992
|g number:1
|g day:15
|g month:03
|g pages:84-91
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 24
|j 1992
|e 1
|b 15
|c 03
|h 84-91
|