Comparison of assays for the determination of entomogenous nematode infectivity

Injection, contact, and soil assays were used to compare infectivity of Heterorhabditis bacteriophora strain HP88 and Steinernema carpocapsae strain All to final instar Galleria mellonella larvae. Under comparable assay conditions, H. bacteriophora produced less Galleria mortality and showed greater...

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Détails bibliographiques
Publié dans:Journal of nematology. - 1969. - 24(1992), 2 vom: 15. Juni, Seite 271-4
Auteur principal: Sims, S R (Auteur)
Autres auteurs: Downing, A S, Pershing, J C
Format: Article
Langue:English
Publié: 1992
Accès à la collection:Journal of nematology
Sujets:Journal Article Bioassay Galleria mellonella Heterorhabditis bacteriophora Steinernema carpocapsae infectivity assay injection nematode
Description
Résumé:Injection, contact, and soil assays were used to compare infectivity of Heterorhabditis bacteriophora strain HP88 and Steinernema carpocapsae strain All to final instar Galleria mellonella larvae. Under comparable assay conditions, H. bacteriophora produced less Galleria mortality and showed greater within-assay variability in infectivity than S. carpocapsae. Injection of individual S. carpocapsae or H. bacteriophora infective juveniles into Galleria indicated that a comparatively greater percentage of S. carpocapsae was capable of initiating infection. In addition to nematode species, other major components of variability in assay estimations of nematode infectivity were number of nematodes used in the assay, assay type, date of the assay, and possibly, Galleria age
Description:Date Completed 14.07.2011
Date Revised 20.10.2021
published: Print
Citation Status PubMed-not-MEDLINE
ISSN:0022-300X