Comparison of assays for the determination of entomogenous nematode infectivity
Injection, contact, and soil assays were used to compare infectivity of Heterorhabditis bacteriophora strain HP88 and Steinernema carpocapsae strain All to final instar Galleria mellonella larvae. Under comparable assay conditions, H. bacteriophora produced less Galleria mortality and showed greater...
Publié dans: | Journal of nematology. - 1969. - 24(1992), 2 vom: 15. Juni, Seite 271-4 |
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Auteur principal: | |
Autres auteurs: | , |
Format: | Article |
Langue: | English |
Publié: |
1992
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Accès à la collection: | Journal of nematology |
Sujets: | Journal Article Bioassay Galleria mellonella Heterorhabditis bacteriophora Steinernema carpocapsae infectivity assay injection nematode |
Résumé: | Injection, contact, and soil assays were used to compare infectivity of Heterorhabditis bacteriophora strain HP88 and Steinernema carpocapsae strain All to final instar Galleria mellonella larvae. Under comparable assay conditions, H. bacteriophora produced less Galleria mortality and showed greater within-assay variability in infectivity than S. carpocapsae. Injection of individual S. carpocapsae or H. bacteriophora infective juveniles into Galleria indicated that a comparatively greater percentage of S. carpocapsae was capable of initiating infection. In addition to nematode species, other major components of variability in assay estimations of nematode infectivity were number of nematodes used in the assay, assay type, date of the assay, and possibly, Galleria age |
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Description: | Date Completed 14.07.2011 Date Revised 20.10.2021 published: Print Citation Status PubMed-not-MEDLINE |
ISSN: | 0022-300X |