Reproductive Fitness and Random Amplified Polymorphic DNA Variation among Isolates of Pratylenchus vulnus

The reproductive fitness of seven isolates of Pratylenchus vulnus from different geographical areas and hosts was assessed in monoxenic cultures (carrot), and greenhouse cultures (plum, sour orange, and quince). The genetic makeup of the different isolates was compared by Random Amplified Polymorphi...

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Veröffentlicht in:Journal of nematology. - 1969. - 26(1994), 3 vom: 10. Sept., Seite 271-7
1. Verfasser: Pinochet, P (VerfasserIn)
Weitere Verfasser: Cenis, J L, Fernández, C, Doucet, M, Maruli, J
Format: Aufsatz
Sprache:English
Veröffentlicht: 1994
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Pratylenchus vulnus RAPD-PCR genetic variability nematode race reproductive fitness root-lesion nematode
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100 1 |a Pinochet, P  |e verfasserin  |4 aut 
245 1 0 |a Reproductive Fitness and Random Amplified Polymorphic DNA Variation among Isolates of Pratylenchus vulnus 
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520 |a The reproductive fitness of seven isolates of Pratylenchus vulnus from different geographical areas and hosts was assessed in monoxenic cultures (carrot), and greenhouse cultures (plum, sour orange, and quince). The genetic makeup of the different isolates was compared by Random Amplified Polymorphic DNA (RAPD-PCR). The apple (PvAP-S) and apricot (PvAT-F) isolates reproduced less in monoxenic cultures than the rose (PvRO-S) and walnut (PvWA-A and PvWA-U) isolates. On plum, the rose isolate (PvRO-S) reproduced better than the apple (PvAP-S) and walnut isolate from the United States (PvWA-U). On sour orange, the apple (PvAP-S), unknown origin (PvU-UK), and walnut isolate from Argentina (PvWA-A) multiplied well, whereas the walnut isolate from the United States (PvWA-U), apricot (PvAT-F), and rose (PvRO-S) did not. On quince, the apple (PvAP-S) and walnut (PvWA-U) isolates showed a higher reproduction than the one from unknown origin (PvU-UK). RAPD-PCR patterns among the seven P. vulnus isolates were similar, although high intraspecific varibility was detected. Very few bands of P. neglectus were shared by any population of P. vulnus. A high degree of similarity was found among the patterns corresponding to the rose (PvRO-S), apple (PvAP-S), walnut from the United States (PvWA-U), and unknown origin (PvUK-U) isolates. The apricot isolate (PvAT-F) was the most dissimilar among the seven isolates. No correlation could be established between the genetic variation of P. vulnus detected by RAPD-PCR and reproductive fitness. Results demonstrate high genetic varibility between geographically separated populations of P. vulnus 
650 4 |a Journal Article 
650 4 |a Pratylenchus vulnus 
650 4 |a RAPD-PCR 
650 4 |a genetic variability 
650 4 |a nematode 
650 4 |a race 
650 4 |a reproductive fitness 
650 4 |a root-lesion nematode 
700 1 |a Cenis, J L  |e verfasserin  |4 aut 
700 1 |a Fernández, C  |e verfasserin  |4 aut 
700 1 |a Doucet, M  |e verfasserin  |4 aut 
700 1 |a Maruli, J  |e verfasserin  |4 aut 
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