Characterization of Heterorhabditis Isolates by PCR Amplification of Segments of mtDNA and rDNA Genes

Restriction digests of amplified DNA from the mitochondrial genome and the nuclear ribosomal internally transcribed spacer region have been evaluated as genetic markers for species groups in Heterorhabditis. Six RFLP profiles have been identified. These profiles supported groupings determined by cro...

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Veröffentlicht in:Journal of nematology. - 1969. - 26(1994), 3 vom: 10. Sept., Seite 260-70
1. Verfasser: Joyce, S A (VerfasserIn)
Weitere Verfasser: Burnell, A M, Powers, T O
Format: Aufsatz
Sprache:English
Veröffentlicht: 1994
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Heterorhabditis entomopathogenic nematode molecular diagnostics mtDNA nematode ribosomal DNA
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245 1 0 |a Characterization of Heterorhabditis Isolates by PCR Amplification of Segments of mtDNA and rDNA Genes 
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520 |a Restriction digests of amplified DNA from the mitochondrial genome and the nuclear ribosomal internally transcribed spacer region have been evaluated as genetic markers for species groups in Heterorhabditis. Six RFLP profiles have been identified. These profiles supported groupings determined by cross-breeding studies and were in agreement with less definitive groupings based on other biochemical and molecular methods. Digestion patterns of both amplification products provided strong evidence for the recognition of species groups, which include Irish, NW European, tropical, and a H. bacteriophora complex. The H. bacteriophora complex could be further resolved into three genotypes represented by H. zealandica, the H. bacteriophora, Brecon (Australian) type isolate for H. bacteriophora, and a grouping composed of isolates NC1, V16, HI82, and HP88. All cultures obtained of the H. megidis isolate were identical to the NW European group. These results could be used to aid monitoring of field release of Heterorhabditis as well as allowing a rapid initial assessment of taxonomic grouping 
650 4 |a Journal Article 
650 4 |a Heterorhabditis 
650 4 |a entomopathogenic nematode 
650 4 |a molecular diagnostics 
650 4 |a mtDNA 
650 4 |a nematode 
650 4 |a ribosomal DNA 
700 1 |a Burnell, A M  |e verfasserin  |4 aut 
700 1 |a Powers, T O  |e verfasserin  |4 aut 
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