Phenotypic and Genetic Characterization of Two New Mutants of Heterorhabditis bacteriophora

Two new "dumpy" mutants (Hbdpy-2 and Hbdpy-3) of Heterorhabditis bacteriophora were induced and characterized. Mutants (hermaphrodites and males) that hatched from eggs were shorter and wider than the wild-type strain. This phenotype was not discernible in young animals until 24 hours afte...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Journal of nematology. - 1969. - 26(1994), 1 vom: 10. März, Seite 32-9
1. Verfasser: Koltai, H (VerfasserIn)
Weitere Verfasser: Glazer, I, Segal, D
Format: Aufsatz
Sprache:English
Veröffentlicht: 1994
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Heterorhabditis bacteriophora dumpy mutant genetics linkage map mutagenesis nematode
LEADER 01000caa a22002652c 4500
001 NLM187023018
003 DE-627
005 20250210061926.0
007 tu
008 231223s1994 xx ||||| 00| ||eng c
028 5 2 |a pubmed25n0623.xml 
035 |a (DE-627)NLM187023018 
035 |a (NLM)19279866 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Koltai, H  |e verfasserin  |4 aut 
245 1 0 |a Phenotypic and Genetic Characterization of Two New Mutants of Heterorhabditis bacteriophora 
264 1 |c 1994 
336 |a Text  |b txt  |2 rdacontent 
337 |a ohne Hilfsmittel zu benutzen  |b n  |2 rdamedia 
338 |a Band  |b nc  |2 rdacarrier 
500 |a Date Completed 14.07.2011 
500 |a Date Revised 20.10.2021 
500 |a published: Print 
500 |a Citation Status PubMed-not-MEDLINE 
520 |a Two new "dumpy" mutants (Hbdpy-2 and Hbdpy-3) of Heterorhabditis bacteriophora were induced and characterized. Mutants (hermaphrodites and males) that hatched from eggs were shorter and wider than the wild-type strain. This phenotype was not discernible in young animals until 24 hours after hatching from eggs or in mutants that developed from infective juveniles. Scanning electron microscopy revealed that the tails of the two mutants are much more slender than in the wild-type. In addition, the vulva of Hbdpy-3 nematodes appeared to be sunken; that of Hbdpy-2 animals was protruding, like in the wild-type. Upon self fertilization, individual Hbdpy-3 hermaphrodites produced fewer progeny than the wild-type. Crosses between virgin Hbdpy-2 and Hbdpy-3 hermaphrodites and wild-type males indicated that the two mutations are recessive. Complementation tests indicated that Hbdpy-1, Hbdpy-2, and Hbdpy-3 affect different genes. The ratio (1.03:1) of wild-type to dumpy phenotype among the F progeny of self-fertilizing heterozygotes suggested linkage among the three genes. The genetic map distance was estimated only between Hbdpy-1 and Hbdpy-2 genes, approximately 29 map units 
650 4 |a Journal Article 
650 4 |a Heterorhabditis bacteriophora 
650 4 |a dumpy mutant 
650 4 |a genetics 
650 4 |a linkage map 
650 4 |a mutagenesis 
650 4 |a nematode 
700 1 |a Glazer, I  |e verfasserin  |4 aut 
700 1 |a Segal, D  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of nematology  |d 1969  |g 26(1994), 1 vom: 10. März, Seite 32-9  |w (DE-627)NLM098196421  |x 0022-300X  |7 nnas 
773 1 8 |g volume:26  |g year:1994  |g number:1  |g day:10  |g month:03  |g pages:32-9 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 26  |j 1994  |e 1  |b 10  |c 03  |h 32-9