Biotin-Avidin ELISA Detection of Grapevine Fanleaf Virus in the Vector Nematode Xiphinema index

The value of biotin-avidin (B-A) ELISA for the detection of grapevine fanleaf virus (GFLV) in Xiphinema was estimated with field populations and greenhouse subpopulations. Samples consisted of increasing numbers of adults ranging from 1 to 64 in multiples of two. Tests with virus-free X. index popul...

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Veröffentlicht in:Journal of nematology. - 1969. - 25(1993), 3 vom: 10. Sept., Seite 401-5
1. Verfasser: Esmenjaud, D (VerfasserIn)
Weitere Verfasser: Walter, B, Minot, J C, Voisin, R, Cornuet, P
Format: Aufsatz
Sprache:English
Veröffentlicht: 1993
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article ELISA X. pachtaicum Xiphinema index diagnosis grape fanleaf virus nematode virus
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245 1 0 |a Biotin-Avidin ELISA Detection of Grapevine Fanleaf Virus in the Vector Nematode Xiphinema index 
264 1 |c 1993 
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520 |a The value of biotin-avidin (B-A) ELISA for the detection of grapevine fanleaf virus (GFLV) in Xiphinema was estimated with field populations and greenhouse subpopulations. Samples consisted of increasing numbers of adults ranging from 1 to 64 in multiples of two. Tests with virus-free X. index populations reared on grapevine and fig plants as negative controls did not reveal a noticeable effect of the host plant. ELISA absorbances of virus-free X. index samples were greater than corresponding absorbances of X. pachtaicum samples. Differences occurred between two X. index field populations from GFLV-infected grapevines in Champagne and Languedoc. In most tests, 1-, 2-, 4-, and 8-nematode samples of virus-free and virus-infected populations, respectively, could not be separated. Consequently, B-A ELISA was not a reliable method for GFLV detection in samples of less than 10 X. index adults, but comparison of the absorbances obtained with increasing numbers may allow differentiation of the viral infectious potential of several populations 
650 4 |a Journal Article 
650 4 |a ELISA 
650 4 |a X. pachtaicum 
650 4 |a Xiphinema index 
650 4 |a diagnosis 
650 4 |a grape fanleaf virus 
650 4 |a nematode 
650 4 |a virus 
700 1 |a Walter, B  |e verfasserin  |4 aut 
700 1 |a Minot, J C  |e verfasserin  |4 aut 
700 1 |a Voisin, R  |e verfasserin  |4 aut 
700 1 |a Cornuet, P  |e verfasserin  |4 aut 
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