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|a pubmed25n0623.xml
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|a (DE-627)NLM187011060
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|a (NLM)19278588
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a chi
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| 100 |
1 |
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|a Fang, Wei
|e verfasserin
|4 aut
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| 245 |
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|a Effect of C5a on expression of thrombomodulin in endothelial cells in vitro
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|c 2009
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 11.05.2010
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|a Date Revised 12.03.2009
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a OBJECTIVE: To investigate the effect of C5a on the expression of thrombomodulin (TM) in human umbilical vein endothelial cells (HUVECs)
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|a METHODS: HUVECs cultured in vitro were stimulated with C5a for 8, 12, 16, 20 hours in a concentration of 200 microg/L, and also with different concentrations of 100, 200, 300 microg/L for 12 hours respectively. The levels of both mRNA and protein expression of TM was detected by real-time polymerase chain reaction (PCR) and Western blotting respectively, and the dose and time dependent effects of C5a on the expression of TM were evaluated
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|a RESULTS: C5a down-regulated the TM expression at both protein and mRNA level. The down-regulation was time-dependent [(93.11+/- 1.57) x10(-2) , (71.05+/-3.39)x10(-2), (65.48+/-4.28)x10(-2), (62.69+/-4.03)x10(-2)at protein level and (301.71+/-80.40)x10(-6), (38.29+/-20.24)x10(-6), (8.82+/-2.66)x10(-6), (7.05+/-0.80)x10(-6) at mRNA level, all P<0.05] and dose-dependent [(113.25+/-3.97)x10(-2), (80.18+/-2.56)x10(-2), (73.22+/- 4.36)x10(-2) at protein level and (401.77+/-20.46)x10(-6), (31.12+/-3.51)x10(-6), (18.19+/-1.46)x10(-6) at mRNA level , all P<0.05]. When concentration of C5a at 300 microg/L was used to stimulate HUVECs for longer than 12 hours, the lowering of TM at protein level was slowed down obviously. And concentration of C5a at 200 microg/L was used to stimulate HUVECs for 12 hours, the lowering of TM at mRNA level was slowed down obviously
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|a CONCLUSION: C5a can depress the gene expression of TM, and then affect the protein's translation. By this means, C5a can lead to hypercoagulability and inflammatory injuries in sepsis
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|a English Abstract
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 650 |
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|a Thrombomodulin
|2 NLM
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| 650 |
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|a Complement C5a
|2 NLM
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| 650 |
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|a 80295-54-1
|2 NLM
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| 700 |
1 |
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|a Guo, Zhen-hui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Bao-quan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Xiao-feng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Ping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lv, Feng-lin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Su, Lei
|e verfasserin
|4 aut
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| 773 |
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8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 21(2009), 3 vom: 12. März, Seite 168-71
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
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|g volume:21
|g year:2009
|g number:3
|g day:12
|g month:03
|g pages:168-71
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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|d 21
|j 2009
|e 3
|b 12
|c 03
|h 168-71
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