Mica surface promotes the assembly of cytoskeletal proteins

We report the surface-mediated polymerization of FtsZ protein, the prokaryote homologue of tubulin, by AFM. FtsZ protein can form filaments on mica whereas the bulk FtsZ concentration is orders of magnitude lower than the critical concentration. Surface polymerization is favored by a local increase...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 25(2009), 6 vom: 09. Apr., Seite 3331-5
1. Verfasser: Hamon, Loic (VerfasserIn)
Weitere Verfasser: Panda, Dulal, Savarin, Philippe, Joshi, Vandana, Bernhard, Johann, Mucher, Elodie, Mechulam, Alain, Curmi, Patrick A, Pastré, David
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Aluminum Silicates Bacterial Proteins Cytoskeletal Proteins FtsZ protein, Bacteria Polymers Tubulin Glycerol PDC6A3C0OX mehr... mica V8A1AW0880
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520 |a We report the surface-mediated polymerization of FtsZ protein, the prokaryote homologue of tubulin, by AFM. FtsZ protein can form filaments on mica whereas the bulk FtsZ concentration is orders of magnitude lower than the critical concentration. Surface polymerization is favored by a local increase in protein concentration and requires a high mobility of proteins on the surface. To generalize to other cytoskeleton protein, we also show that mica can initiate the formation of tubulin protofilaments. This study is of particular interest for studying cytoskeletal protein dynamics by AFM but also for the surface autoassembly of nanostructures 
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650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Bacterial Proteins  |2 NLM 
650 7 |a Cytoskeletal Proteins  |2 NLM 
650 7 |a FtsZ protein, Bacteria  |2 NLM 
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700 1 |a Panda, Dulal  |e verfasserin  |4 aut 
700 1 |a Savarin, Philippe  |e verfasserin  |4 aut 
700 1 |a Joshi, Vandana  |e verfasserin  |4 aut 
700 1 |a Bernhard, Johann  |e verfasserin  |4 aut 
700 1 |a Mucher, Elodie  |e verfasserin  |4 aut 
700 1 |a Mechulam, Alain  |e verfasserin  |4 aut 
700 1 |a Curmi, Patrick A  |e verfasserin  |4 aut 
700 1 |a Pastré, David  |e verfasserin  |4 aut 
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