In-situ Hybridization to Messenger RNA in Heterodera glycines
A method is presented for in-situ hybridization to mRNA in second-stage juveniles (J2) of the soybean cyst nematode Heterodera glycines. The protocol was developed using a digoxigenin-labeled RNA probe transcribed from cDNA of a cellulase gene that was known to be expressed in the subventral esophag...
| Veröffentlicht in: | Journal of nematology. - 1969. - 30(1998), 3 vom: 09. Sept., Seite 309-12 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1998
|
| Zugriff auf das übergeordnete Werk: | Journal of nematology |
| Schlagworte: | Journal Article Heterodera glycines cellulase gene digoxigenin RNA probe esophageal gland in-situ hybridization nematode |