The rDNA Internal Transcribed Spacer Region as a Taxonomic Marker for Nematodes

The ITS region from a wide taxonomic range of nematodes, including secernentean and adenophorean taxa, and free-living, entomopathogenic, and plant-parasitic species, was evaluated as a taxonomic marker. Size of the amplified product aided in the initial determination of group membership, and also s...

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Détails bibliographiques
Publié dans:Journal of nematology. - 1969. - 29(1997), 4 vom: 09. Dez., Seite 441-50
Auteur principal: Powers, T O (Auteur)
Autres auteurs: Todd, T C, Burnell, A M, Murray, P C, Fleming, C C, Szalanski, A L, Adams, B A, Harris, T S
Format: Article
Langue:English
Publié: 1997
Accès à la collection:Journal of nematology
Sujets:Journal Article ITS diagnosis genetics internal transcribed spacer region molecular ecology nematode systematics taxonomy
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245 1 4 |a The rDNA Internal Transcribed Spacer Region as a Taxonomic Marker for Nematodes 
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520 |a The ITS region from a wide taxonomic range of nematodes, including secernentean and adenophorean taxa, and free-living, entomopathogenic, and plant-parasitic species, was evaluated as a taxonomic marker. Size of the amplified product aided in the initial determination of group membership, and also suggested groups that may require taxonomic reevaluation. Congeneric species often displayed identically sized ITS regions, but genera such as Pratylenchus and Tylenchorhynchus had species with large differences in size. ITS heterogeneity in individuals and populations was identified in several nematode taxa. PCR-RFLP of ITS1 is advocated as a method of taxonomic analysis in genera such as Helicotylenchus that contain numerous species with few diagnostic morphological characteristics 
650 4 |a Journal Article 
650 4 |a ITS 
650 4 |a diagnosis 
650 4 |a genetics 
650 4 |a internal transcribed spacer region 
650 4 |a molecular ecology 
650 4 |a nematode 
650 4 |a systematics 
650 4 |a taxonomy 
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700 1 |a Burnell, A M  |e verfasserin  |4 aut 
700 1 |a Murray, P C  |e verfasserin  |4 aut 
700 1 |a Fleming, C C  |e verfasserin  |4 aut 
700 1 |a Szalanski, A L  |e verfasserin  |4 aut 
700 1 |a Adams, B A  |e verfasserin  |4 aut 
700 1 |a Harris, T S  |e verfasserin  |4 aut 
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