Differences in Hatching of Heterodera glycines Egg-mass and Encysted Eggs in vitro

Hatching studies with Heterodera glycines typically have been conducted with a mixture of egg-mass and encysted eggs. Laboratory research was conducted to compare hatching of H. glycines eggs from external egg masses with that of eggs extracted from within females and cysts (encysted eggs). Egg-mass...

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Veröffentlicht in:Journal of nematology. - 1969. - 29(1997), 3 vom: 09. Sept., Seite 315-21
1. Verfasser: Thompson, J M (VerfasserIn)
Weitere Verfasser: Tylka, G L
Format: Aufsatz
Sprache:English
Veröffentlicht: 1997
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Heterodera glycines ecology egg mass embryogeny encysted eggs flow cytometry hatch assay hatch inhibitor hatching soybean cyst nematode
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245 1 0 |a Differences in Hatching of Heterodera glycines Egg-mass and Encysted Eggs in vitro 
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520 |a Hatching studies with Heterodera glycines typically have been conducted with a mixture of egg-mass and encysted eggs. Laboratory research was conducted to compare hatching of H. glycines eggs from external egg masses with that of eggs extracted from within females and cysts (encysted eggs). Egg-mass eggs were collected by soaking infected soybean roots in 0.5% sodium hypochlorite, and encysted eggs were collected from females and cysts dislodged from the same roots with a stream of water. Eggs were incubated at 25 degrees C in deionized water, 3.0 mM ZnSOsolution, or one of three synthetic H. glycines hatch inhibitors, mad hatched juveniles were counted every other day for 22 days. Samples of eggs collected at the beginning and end of all experiments were analyzed to determine extent of embryo development. Egg-mass eggs hatched more rapidly than encysted eggs during the first 16 days, but not thereafter. Throughout the experiments, hatch of egg-mass eggs in deionized water was greater than that of encysted eggs. From day 8 to day 22, egg-mass eggs were less sensitive than encysted eggs to the hatch inhibitor 2-(2'-carboxyethyl)-5-[carboxy(hydroxy)methylidenyl]cyclopentanone. A greater proportion of egg-mass eggs contained vermiform juveniles than did encysted eggs at the beginning of the experiments, but not at the end. Results indicated that H. glycines egg-mass and encysted eggs have different hatching behaviors that cannot be explained entirely by differences in embryological development 
650 4 |a Journal Article 
650 4 |a Heterodera glycines 
650 4 |a ecology 
650 4 |a egg mass 
650 4 |a embryogeny 
650 4 |a encysted eggs 
650 4 |a flow cytometry 
650 4 |a hatch assay 
650 4 |a hatch inhibitor 
650 4 |a hatching 
650 4 |a soybean cyst nematode 
700 1 |a Tylka, G L  |e verfasserin  |4 aut 
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