DNA sequences from formalin-fixed nematodes : integrating molecular and morphological approaches to taxonomy

To effectively integrate DNA sequence analysis and classical nematode taxonomy, we must be able to obtain DNA sequences from formalin-fixed specimens. Microdissected sections of nematodes were removed from specimens fixed in formalin, using standard protocols and without destroying morphological fea...

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Détails bibliographiques
Publié dans:Journal of nematology. - 1969. - 29(1997), 3 vom: 09. Sept., Seite 250-4
Auteur principal: Thomas, W K (Auteur)
Autres auteurs: Vida, J T, Frisse, L M, Mundo, M, Baldwin, J G
Format: Article
Langue:English
Publié: 1997
Accès à la collection:Journal of nematology
Sujets:Journal Article DNA sequence formalin microdissection molecular biology nematode polymerase chain reaction taxonomy
Description
Résumé:To effectively integrate DNA sequence analysis and classical nematode taxonomy, we must be able to obtain DNA sequences from formalin-fixed specimens. Microdissected sections of nematodes were removed from specimens fixed in formalin, using standard protocols and without destroying morphological features. The fixed sections provided sufficient template for multiple polymerase chain reaction-based DNA sequence analyses
Description:Date Completed 14.07.2011
Date Revised 20.10.2021
published: Print
Citation Status PubMed-not-MEDLINE
ISSN:0022-300X