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01000caa a22002652c 4500 |
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NLM186969740 |
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DE-627 |
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20250210060932.0 |
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tu |
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231223s1997 xx ||||| 00| ||eng c |
| 028 |
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2 |
|a pubmed25n0623.xml
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|a (DE-627)NLM186969740
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| 035 |
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|a (NLM)19274151
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Stetina, S R
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Extraction of Root-associated Meloidogyne incognita and Rotylenchulus reniformis
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| 264 |
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1 |
|c 1997
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 14.07.2011
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| 500 |
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|a Date Revised 20.10.2021
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a A technique based on physical maceration of root tissue was developed to extract vermiform and swollen stages of Meloidogyne incognita and Rotylenchulus reniformis. Experiments conducted on soybean and tomato evaluated the efficiency of method (stir, grind), NaOC1 concentration (0%, 0.5%), and duration (lx, 2x) on extraction of nematodes and eggs from 60-day-old populations. Root-associated populations of R. reniformis were considerably lower than those of M. incognita, so development of the method focused on the latter. Grinding liberated more nematodes than stirring, but the reverse was true for egg extraction. Among grinding treatments, a duration of 10 seconds in 0.5% NaOCl provided the most efficient extraction of nematodes and eggs. Among stirring treatments, a duration of 10 minutes in 0.5% NaOCl provided the most efficient extraction of eggs. These techniques were compared on soybean roots 30 days older than those on which the procedures were first evaluated, with consistent results
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Meloidogyne incognita
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| 650 |
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4 |
|a Rotylenchulus reniformis
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| 650 |
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4 |
|a extraction
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| 650 |
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4 |
|a grinding
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| 650 |
|
4 |
|a maceration
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| 650 |
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4 |
|a method
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| 650 |
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4 |
|a nematode
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| 650 |
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4 |
|a reniform nematode
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| 650 |
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4 |
|a root-knot nematode
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| 700 |
1 |
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|a McGawley, E C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Russin, J S
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of nematology
|d 1969
|g 29(1997), 2 vom: 09. Juni, Seite 209-15
|w (DE-627)NLM098196421
|x 0022-300X
|7 nnas
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| 773 |
1 |
8 |
|g volume:29
|g year:1997
|g number:2
|g day:09
|g month:06
|g pages:209-15
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 29
|j 1997
|e 2
|b 09
|c 06
|h 209-15
|