Comparison of Extraction and Shipping Methods for Cysts and Juveniles of Heterodera glycines

Experiments to determine the effects of extraction techniques and the influence of shipping on extraction of Heterodera glycines life stages gave variable results. Shipping did not significantly affect numbers of nematodes extracted. More second-stage juveniles (J2) were extracted with Baermann funn...

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Bibliographische Detailangaben
Veröffentlicht in:Journal of nematology. - 1969. - 29(1997), 1 vom: 09. März, Seite 127-32
1. Verfasser: Riggs, R D (VerfasserIn)
Weitere Verfasser: Schmitt, D P, Mauromoustakos, A
Format: Aufsatz
Sprache:English
Veröffentlicht: 1997
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Baennann funnel Heterodera glycines centrifugal flotation cyst egg elutriation extraction methods nematode mehr... second-stage juvenile shipping soybean cyst nematode
Beschreibung
Zusammenfassung:Experiments to determine the effects of extraction techniques and the influence of shipping on extraction of Heterodera glycines life stages gave variable results. Shipping did not significantly affect numbers of nematodes extracted. More second-stage juveniles (J2) were extracted with Baermann funnels than with an elutriator, probably because incubation of encysted eggs on the Baermann funnel for 1 week allowed hatching to occur. Sieving was more efficient than elutriation for extracting cysts. Adding air agitation to the water pressure during elutriation increased extraction efficiency of cysts but not J2. Sample sizes of 250 cm(3) and 500 cm(3) did not influence extraction efficiency of cysts; however, sample size did influence extraction of J2
Beschreibung:Date Completed 14.07.2011
Date Revised 20.10.2021
published: Print
Citation Status PubMed-not-MEDLINE
ISSN:0022-300X