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NLM186939582 |
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DE-627 |
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20231223175053.0 |
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231223s2000 xx ||||| 00| ||eng c |
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|a pubmed24n0623.xml
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|a (DE-627)NLM186939582
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|a (NLM)19270966
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Wergin, W P
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Effect of an ice-nucleating activity agent on subzero survival of nematode juveniles
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| 264 |
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1 |
|c 2000
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 14.07.2011
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| 500 |
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|a Date Revised 20.10.2021
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a Juveniles of five species of nematodes, Caenorhabditis elegans, Panagrellus redivivus, Pratylenchus agilis, Pristionchus pacificus, and Distolabrellus veechi, were added to solutions with (treatment) and without (control) a commercial ice-nucleating activity (INA) agent. Ten-microliter droplets of the solutions containing the juveniles were placed on glass microscope slides and transferred to a temperaturecontrolled freeze plate where the temperature was reduced to -6 to -8 degrees C. At this temperature, the droplets containing the INA agent froze while those without the agent remained liquid. After 2 minutes, the temperature of the plate was raised to 24 degrees C, and the slides were examined with a light microscope to determine the viability of the juveniles. The results showed that usually most juveniles (43% to 88%, depending on species) in solutions that did not contain the INA agent (controls) were active, indicating that the juveniles were capable of supercooling and were thereby protected from the subzero temperatures. Alternatively, less than 10% of the juveniles that had frozen for 2 minutes in solutions containing the INA agent remained viable, indicating that inoculative freezing of the solution was lethal to the supercooled juveniles. Our results suggest that, in geographical areas where winter temperatures may not be sufficiently low or sustained to freeze soil, the addition of an INA agent may help induce ice nucleation and thereby reduce the populations of nematode species that are unable to survive when the soil solution is frozen
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|a Journal Article
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|a Caenorhabditis elegans
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| 650 |
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4 |
|a Distolabrellus veechi
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| 650 |
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4 |
|a INA
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| 650 |
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4 |
|a Panagrellus redivivus
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| 650 |
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4 |
|a Pratylenchus agilis
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| 650 |
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4 |
|a Pristionchus pacificus
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| 650 |
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4 |
|a Snomax
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| 650 |
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4 |
|a cryobiology
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| 650 |
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4 |
|a freeze susceptibility
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| 650 |
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4 |
|a freeze tolerance
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| 650 |
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4 |
|a ice-nucleating activity agent
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| 650 |
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4 |
|a nematode
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| 650 |
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4 |
|a supercooling
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| 700 |
1 |
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|a Yaklich, R W
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Carta, L K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Erbe, E F
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Murphy, C A
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of nematology
|d 1969
|g 32(2000), 2 vom: 16. Juni, Seite 198-204
|w (DE-627)NLM098196421
|x 0022-300X
|7 nnns
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| 773 |
1 |
8 |
|g volume:32
|g year:2000
|g number:2
|g day:16
|g month:06
|g pages:198-204
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 32
|j 2000
|e 2
|b 16
|c 06
|h 198-204
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