FMRFamide-like Immunoactivity in Heterodera glycines (Nemata : Tylenchida)

Material antigenically related to the neuromodulatory peptide FMRFamide was detected and examined in preparations of the soybean cyst nematode, Heterodera glycines, and in the free-living nematodes Caenorhabditis elegans and Panagrellus redivivus. FMRFamide-related peptides were quantified by an enz...

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Veröffentlicht in:Journal of nematology. - 1969. - 31(1999), 2 vom: 16. Juni, Seite 224-31
1. Verfasser: Masler, E P (VerfasserIn)
Weitere Verfasser: Kovaleva, E S, Sardanelli, S
Format: Aufsatz
Sprache:English
Veröffentlicht: 1999
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Caenorhabditis elegans ELISA FMRFamide FaRP Heterodera glycines Panagrellus redivivus RP-HPLC female aging juvenile development mehr... nematode peptide soybean cyst nematode
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245 1 0 |a FMRFamide-like Immunoactivity in Heterodera glycines (Nemata  |b Tylenchida) 
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520 |a Material antigenically related to the neuromodulatory peptide FMRFamide was detected and examined in preparations of the soybean cyst nematode, Heterodera glycines, and in the free-living nematodes Caenorhabditis elegans and Panagrellus redivivus. FMRFamide-related peptides were quantified by an enzyme-linked immunosorbent assay. Specific activities were remarkably similar among all of the vermiform members of the three species. FMRFamide-related peptide immunoactivity was present in both sexes and all stages of H. glycines examined. The highest specific activity was present in second-stage juveniles and in males, and the lowest in white and yellow females. Total FMRFamide-related peptide level per individual was highest in brown females, with 90% of the activity associated with the eggs. Peptide levels in these eggs and in second-stage juveniles were comparable and increased in adults, especially in females. Chromatographic analysis of FMRFamide-related peptide preparations from H. glycines juveniles, C. elegans, and P. redivivus revealed distinct qualitative differences between the infective plant parasite and the free-living nematodes 
650 4 |a Journal Article 
650 4 |a Caenorhabditis elegans 
650 4 |a ELISA 
650 4 |a FMRFamide 
650 4 |a FaRP 
650 4 |a Heterodera glycines 
650 4 |a Panagrellus redivivus 
650 4 |a RP-HPLC 
650 4 |a female aging 
650 4 |a juvenile development 
650 4 |a nematode 
650 4 |a peptide 
650 4 |a soybean cyst nematode 
700 1 |a Kovaleva, E S  |e verfasserin  |4 aut 
700 1 |a Sardanelli, S  |e verfasserin  |4 aut 
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