Identification of Seed Gall Nematodes of Agronomic and Regulatory Concern with PCR-RFLP of ITS1

A molecular analysis of eight described species of seed gall nematode, along with six undescribed isolates from different hosts, has revealed a strong association between nucleotide sequence polymorphism and host status. Each anguinid nematode associated with a unique host produced a unique PCR-RFLP...

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Veröffentlicht in:Journal of nematology. - 1969. - 33(2001), 4 vom: 24. Dez., Seite 191-4
1. Verfasser: Powers, T O (VerfasserIn)
Weitere Verfasser: Szalanski, A L, Mullin, P G, Harris, T S, Bertozzi, T, Griesbach, J A
Format: Aufsatz
Sprache:English
Veröffentlicht: 2001
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Afrina wevelli Anguina agrostis Anguina tritici PCR-RFLP diagnostics nematode identification regulatory nematology seed gall nematode
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245 1 0 |a Identification of Seed Gall Nematodes of Agronomic and Regulatory Concern with PCR-RFLP of ITS1 
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520 |a A molecular analysis of eight described species of seed gall nematode, along with six undescribed isolates from different hosts, has revealed a strong association between nucleotide sequence polymorphism and host status. Each anguinid nematode associated with a unique host produced a unique PCR-RFLP pattern for the ITS1 region. Anguina species that had been synonymized in the past, Anguina agrostis, A. funesta, and A. wevelli (Afrina wevelli), were readily discriminated. Two undescribed species from northern New South Wales and southeastern South Australia, reported to be vectors of Rathyaibacter toxicus in the disease called ''floodplain staggers,'' were differentiated by a single restriction enzyme, and both could be separated easily from A. funesta, the vector of R. toxicus in annual ryegrass toxicity. Other species differentiated in this study include A. agropyronifloris, A. graminis, A. microlaenae, A. pacificae, and undescribed species from host species Dactylis glomerata, Agrostis avenacea, Polypogon monospeliensis, Stipa sp., Astrebla pectinata, and Holcus lanatus. Phylogenetic analysis of the ITS1 region suggests that considerable anguinid genetic diversification has accompanied specialization on different host species 
650 4 |a Journal Article 
650 4 |a Afrina wevelli 
650 4 |a Anguina agrostis 
650 4 |a Anguina tritici 
650 4 |a PCR-RFLP 
650 4 |a diagnostics 
650 4 |a nematode identification 
650 4 |a regulatory nematology 
650 4 |a seed gall nematode 
700 1 |a Szalanski, A L  |e verfasserin  |4 aut 
700 1 |a Mullin, P G  |e verfasserin  |4 aut 
700 1 |a Harris, T S  |e verfasserin  |4 aut 
700 1 |a Bertozzi, T  |e verfasserin  |4 aut 
700 1 |a Griesbach, J A  |e verfasserin  |4 aut 
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