Normalization of qRT-PCR data : the necessity of adopting a systematic, experimental conditions-specific, validation of references
Quantitative RT-PCR (reverse transcription polymerase chain reaction, also known as qRT-PCR or real-time RT-PCR) has been used in large proportions of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization u...
Ausführliche Beschreibung
Bibliographische Detailangaben
Veröffentlicht in: | Journal of experimental botany. - 1985. - 60(2009), 2 vom: 05., Seite 487-93
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1. Verfasser: |
Guénin, Stéphanie
(VerfasserIn) |
Weitere Verfasser: |
Mauriat, Mélanie,
Pelloux, Jérôme,
Van Wuytswinkel, Olivier,
Bellini, Catherine,
Gutierrez, Laurent |
Format: | Online-Aufsatz
|
Sprache: | English |
Veröffentlicht: |
2009
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Zugriff auf das übergeordnete Werk: | Journal of experimental botany
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Schlagworte: | Journal Article
Review |