Normalization of qRT-PCR data : the necessity of adopting a systematic, experimental conditions-specific, validation of references

Quantitative RT-PCR (reverse transcription polymerase chain reaction, also known as qRT-PCR or real-time RT-PCR) has been used in large proportions of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization u...

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Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 60(2009), 2 vom: 05., Seite 487-93
1. Verfasser: Guénin, Stéphanie (VerfasserIn)
Weitere Verfasser: Mauriat, Mélanie, Pelloux, Jérôme, Van Wuytswinkel, Olivier, Bellini, Catherine, Gutierrez, Laurent
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Review