Morphological and Molecular Evaluation of a Meloidogyne hapla Population Damaging Coffee (Coffea arabica) in Maui, Hawaii

An unusual population of Meloidogyne hapla, earlier thought to be an undescribed species, was found causing large galls, without adventitious roots, and substantial damage to coffee in Maui, Hawaii. Only in Brazil had similar damage to coffee been reported by this species. Unlike M. exigua from Sout...

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Veröffentlicht in:Journal of nematology. - 1969. - 37(2005), 2 vom: 03. Juni, Seite 136-45
1. Verfasser: Handoo, Z A (VerfasserIn)
Weitere Verfasser: Skantar, A M, Carta, L K, Schmitt, D P
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Coffea arabica Coffea robusta India M. incognita M. javanica M. konaensis Meloidogyne exigua climate detection mehr... identification molecular diagnostics nematode netherlands resistance taxonomy
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245 1 0 |a Morphological and Molecular Evaluation of a Meloidogyne hapla Population Damaging Coffee (Coffea arabica) in Maui, Hawaii 
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520 |a An unusual population of Meloidogyne hapla, earlier thought to be an undescribed species, was found causing large galls, without adventitious roots, and substantial damage to coffee in Maui, Hawaii. Only in Brazil had similar damage to coffee been reported by this species. Unlike M. exigua from South and Central America, this population reproduced well on coffee cv. Mokka and M. incognita-susceptible tomato but poorly on tomato with the Mi resistance gene. Characterization included SEM images, esterase isozymes, and five DNA sequences: i) the D3 segment of the large subunit (LSU-D3 or 28S) rDNA, ii) internal transcribed spacer (ITS-1) rDNA, iii) intergenic spacer (IGS) rDNA, iv) the mitochondrial interval from cytochrome oxidase (CO II) to 16S mtDNA, and v) the nuclear gene Hsp90. Sequences for ITS-1, IGS, and COII were similar to other M. hapla populations, but within species ITS-1 variability was not less than among species. One LSU-D3 haplotype was similar to a previously analyzed population with two minor haplotypes. Hsp90 exhibited some variation between Maryland and Hawaiian populations distinct from other species. Females were narrow with wide vulval slits, large interphasmidial distances, and more posterior excretory pores; 20% of perineal patterns had atypical perivulval lines. Males had a low b ratio (<12 microm). Juveniles had a short distance between stylet and dorsal gland orifice. Juvenile body length was short (<355 microm) and was different between summer and winter populations 
650 4 |a Journal Article 
650 4 |a Coffea arabica 
650 4 |a Coffea robusta 
650 4 |a India 
650 4 |a M. incognita 
650 4 |a M. javanica 
650 4 |a M. konaensis 
650 4 |a Meloidogyne exigua 
650 4 |a climate 
650 4 |a detection 
650 4 |a identification 
650 4 |a molecular diagnostics 
650 4 |a nematode 
650 4 |a netherlands 
650 4 |a resistance 
650 4 |a taxonomy 
700 1 |a Skantar, A M  |e verfasserin  |4 aut 
700 1 |a Carta, L K  |e verfasserin  |4 aut 
700 1 |a Schmitt, D P  |e verfasserin  |4 aut 
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