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231223s2009 xx |||||o 00| ||eng c |
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7 |
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|a 10.1021/la804021t
|2 doi
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|a pubmed24n1324.xml
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|a (DE-627)NLM186779909
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|a (NLM)19253965
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Chen, Yulin
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Fabrication of DNA microarrays with poly(L-glutamic acid) monolayers on gold substrates for SPR imaging measurements
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 02.06.2009
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|a Date Revised 12.03.2024
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|a published: Print
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|a Citation Status MEDLINE
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|a Robust single-stranded DNA (ssDNA) microarrays are created by attaching amine-modified oligonucleotides to a monolayer of poly(L-glutamic acid) (pGlu) that is electrostatically adsorbed onto a chemically modified gold thin film. This surface attachment chemistry methodology is first characterized with a combination of polarization-modulation Fourier transform infrared (PM-FTIR) spectroscopy and surface plasmon resonance (SPR) angle shift measurements. SPR imaging (SPRI) measurements of these ssDNA microarrays are then used to study two surface bioaffinity interactions: (i) the quantitative hybridization adsorption of complementary ssDNA onto mixed ssDNA microarray elements and (ii) the adsorption of single-stranded binding protein (SSB) onto fully and partially hybridized DNA microarray elements. The Langmuir adsorption coefficient (K(Ads)) of SSB binding to ssDNA was determined to be (5.5 +/- 0.4) x 10(9) M(-1)
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Polyglutamic Acid
|2 NLM
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| 650 |
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|a 25513-46-6
|2 NLM
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|a Gold
|2 NLM
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| 650 |
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|a 7440-57-5
|2 NLM
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| 700 |
1 |
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|a Nguyen, Anh
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Niu, Lifang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Corn, Robert M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 25(2009), 9 vom: 05. Mai, Seite 5054-60
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:25
|g year:2009
|g number:9
|g day:05
|g month:05
|g pages:5054-60
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| 856 |
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|u http://dx.doi.org/10.1021/la804021t
|3 Volltext
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