Fluorescence acquisition during hybridization phase in quantitative real-time PCR improves specificity and signal-to-noise ratio

Fluorescence acquisition during hybridization phase in quantitative real-time PCR improves specificity and signal-to-noise ratio

Quantitative real-time PCR (qPCR) is a standard method used for quantification of specific gene expression. This utilizes either dsDNA binding dyes or probe based chemistry. While dsDNA binding dyes have the advantage of low cost and flexibility, fluorescence due to primer dimers also interferes wit...

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Veröffentlicht in:BioTechniques. - 1993. - 45(2008), 6 vom: 25. Dez., Seite 625-6, 628, 630 passim
1. Verfasser: Mehndiratta, Mohit (VerfasserIn)
Weitere Verfasser: Palanichamy, Jayanth Kumar, Ramalingam, Pradeep, Pal, Arnab, Das, Prerna, Sinha, Subrata, Chattopadhyay, Parthaprasad
Format: Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Actins DNA Probes MicroRNAs Oncogene Proteins, Viral RNA, Messenger DNA (Cytosine-5-)-Methyltransferase 1 EC 2.1.1.37 DNA (Cytosine-5-)-Methyltransferases DNMT1 protein, human