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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1021/la8030792
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|a eng
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| 100 |
1 |
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|a Frasca, Guillaume
|e verfasserin
|4 aut
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| 245 |
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|a Formation of a three-dimensional multicellular assembly using magnetic patterning
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|c 2009
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 31.03.2009
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|a Date Revised 09.03.2009
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|a published: Print
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|a Citation Status MEDLINE
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|a We demonstrate a facile approach to design three-dimensional cellular assembly of tunable size and controlled geometry with applications for tissue engineering. Three-dimensional cell patterning was performed using external magnetic forces, without the need for substrate chemical or physical modifications. Human endothelial progenitor cells and mouse macrophages were magnetically labeled using anionic citrate-coated iron oxide nanoparticles. Two magnetic tips were designed, and their magnetic field cartographies were calibrated. The focalized magnetic force generated ensured an efficient entrapment of the cells at the tips vicinity. By tuning the magnetic field gradient geometry and intensity, the magnetic cellular load, and the number of cells, we fully described the formation of the three-dimensional multicellular assemblies, and estimated the corresponding packing factor for a large range of experimental conditions
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|a Journal Article
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|a Gazeau, Florence
|e verfasserin
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| 700 |
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|a Wilhelm, Claire
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 25(2009), 4 vom: 17. Feb., Seite 2348-54
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|g year:2009
|g number:4
|g day:17
|g month:02
|g pages:2348-54
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|u http://dx.doi.org/10.1021/la8030792
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