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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1021/la803556p
|2 doi
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|a pubmed24n0619.xml
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|a (DE-627)NLM185692184
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|a (NLM)19138164
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Kawano, Ryuji
|e verfasserin
|4 aut
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|a Controlling the translocation of single-stranded DNA through alpha-hemolysin ion channels using viscosity
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.02.2009
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a Translocation of single-stranded DNA through alpha-hemolysin (alpha-HL) channels is investigated in glycerol/water mixtures containing 1 M KCl. Experiments using glass nanopore membranes as the lipid bilayer support demonstrate that the translocation velocities of poly(deoxyadenylic acid), poly(deoxycytidylic acid), and poly(deoxythymidylic acid) 50-mers are decreased by a factor of approximately 20 in a 63/37 (vol %) glycerol/water mixture, relative to aqueous solutions. The ion conductance of alpha-HL and the entry rate of the polynucleotides into the protein channel also decrease with increasing viscosity. Precise control of translocation parameters by adjusting viscosity provides a potential means to improve sequencing methods based on ion channel recordings
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a DNA, Single-Stranded
|2 NLM
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|a Hemolysin Proteins
|2 NLM
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|a Ion Channels
|2 NLM
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|a Lipid Bilayers
|2 NLM
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|a Membranes, Artificial
|2 NLM
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|a Polynucleotides
|2 NLM
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|a Water
|2 NLM
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|a Potassium Chloride
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|a Glycerol
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|a PDC6A3C0OX
|2 NLM
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|a Schibel, Anna E P
|e verfasserin
|4 aut
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|a Cauley, Christopher
|e verfasserin
|4 aut
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| 700 |
1 |
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|a White, Henry S
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 25(2009), 2 vom: 20. Jan., Seite 1233-7
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:25
|g year:2009
|g number:2
|g day:20
|g month:01
|g pages:1233-7
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|u http://dx.doi.org/10.1021/la803556p
|3 Volltext
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