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DE-627 |
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231223s2008 xx ||||| 00| ||chi c |
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|a pubmed25n0618.xml
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|a (DE-627)NLM185441769
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|a (NLM)19111114
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a chi
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| 100 |
1 |
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|a Ma, Yan-Xia
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Effect of substance P on expression of beta1-adrenergic receptor in cultured rat cardiomyocytes
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 09.03.2010
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|a Date Revised 29.12.2008
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To investigate the effect of substance P (SP) on expression of beta1-adrenergic receptor (beta1-AR) in cultured neonatal rat cardiomyocytes
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|a METHODS: Cardiomyocytes were obtained from 1-3 days old Sprague-Dawley (SD) rats and cultured for 72 hours. Six wells of the cultured cells were divided into two groups (n=3 in each group): control group and SP group (incubated with 10(-7) mol/L of SP). Immunocytochemistry was used to detect the expression of beta1-AR in cultured rat cardiomyocytes. Fifteen wells of the cultured cells were divided into five groups (n=3 in each group): control group and four SP groups (incubated with 10(-9), 10 (-8), 10(-7), 10(-6) mol/L of SP respectively). Quantitative analysis of the expression of beta1-AR in cultured neonatal rat cardiomyocytes of all the groups was performed using flow cytometry
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|a RESULTS: The results of immunocytochemistry showed that the expression of beta1-AR in cardiomyocytes in SP group was lower than that in control group (positive unit: 179.16+/-48.18 vs. 205.79+/-117.42, average light density: 128.26+/-22.72 vs. 157.35+/-33.11, both P<0.05). The results of flow cytometry showed that significantly lower levels of expression of beta1-AR were observed in the groups treated with SP in the concentrations ranging from 10 (-8) mol/L to 10 (-6) mol/L (151.85+/-4.63,135.00+/-6.84,121.41+/-5.22 vs. 161.35+/-3.09,all P<0.05)
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|a CONCLUSION: SP could down-regulate the expression of beta1-AR in cultured rat cardiomyocytes in vitro
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|a English Abstract
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Receptors, Adrenergic, beta-1
|2 NLM
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| 650 |
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|a Substance P
|2 NLM
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| 650 |
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|a 33507-63-0
|2 NLM
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| 700 |
1 |
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|a Guo, Zheng
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 20(2008), 12 vom: 29. Dez., Seite 710-2
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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|g volume:20
|g year:2008
|g number:12
|g day:29
|g month:12
|g pages:710-2
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