Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

The impacts of the fungicide Opus (epoxiconazole) on marine phytoplankton communities were assessed in a 12-day field experiment using in situ microcosms maintained underwater at 6 m depth. Three community analysis methods were compared for their sensitivity threshold in fungicide impact detection....

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Ecotoxicology (London, England). - 1992. - 18(2009), 3 vom: 15. Apr., Seite 364-76
1. Verfasser: Stachowski-Haberkorn, Sabine (VerfasserIn)
Weitere Verfasser: Quiniou, Louis, Beker, Beatriz, Haberkorn, Hansy, Marie, Dominique, de la Broise, Denis
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Ecotoxicology (London, England)
Schlagworte:Comparative Study Evaluation Study Journal Article Research Support, Non-U.S. Gov't Epoxy Compounds RNA, Ribosomal, 18S Triazoles Xenobiotics epoxiconazole U80T84L776
Beschreibung
Zusammenfassung:The impacts of the fungicide Opus (epoxiconazole) on marine phytoplankton communities were assessed in a 12-day field experiment using in situ microcosms maintained underwater at 6 m depth. Three community analysis methods were compared for their sensitivity threshold in fungicide impact detection. When phytoplankton communities were exposed to 1 microg l(-1) of epoxiconazole, no effects could be demonstrated using TTGE (Temporal Temperature Gradient gel Electrophoresis), flow cytometry or HPLC. When exposed to 10 microg l(-1), TTGE fingerprints from PCR amplified 18S rDNA of communities exhibited significant differences compared with controls (ANOSIM, P = 0.028). Neither flow cytometry counts, nor HPLC pigment profiles allowed to show significant differences in microcosms exposed to 10 microg l(-1) of epoxiconazole. When exposed to 100 microg l(-1), all three methods allowed to detect significant differences in treated microcosms, as compared to controls. The TTGE analysis appears in this study as the most sensitive method for fungicide impact assessment on eukaryote microbial communities
Beschreibung:Date Completed 29.04.2009
Date Revised 20.10.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1573-3017
DOI:10.1007/s10646-008-0288-8