HMA P-type ATPases are the major mechanism for root-to-shoot Cd translocation in Arabidopsis thaliana
The Zn/Cd-transporting ATPases, HMA2 and HMA4, essential for root-to-shoot Zn translocation, are also able to transport Cd. Phytochelatins (PCs) are a major mechanism of Cd detoxification through the sequestration of PC-Cd complexes in vacuoles. The roles of HMA2 and HMA4 in root-to-shoot Cd translo...
| Veröffentlicht in: | The New phytologist. - 1979. - 181(2009), 1 vom: 01., Seite 71-78 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2009
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Arabidopsis Proteins Cadmium 00BH33GNGH Phytochelatins 98726-08-0 Adenosine Triphosphatases EC 3.6.1.- HMA2 protein, Arabidopsis HMA4 protein, Arabidopsis |
| Zusammenfassung: | The Zn/Cd-transporting ATPases, HMA2 and HMA4, essential for root-to-shoot Zn translocation, are also able to transport Cd. Phytochelatins (PCs) are a major mechanism of Cd detoxification through the sequestration of PC-Cd complexes in vacuoles. The roles of HMA2 and HMA4 in root-to-shoot Cd translocation and Cd tolerance were investigated in the PC-deficient, cad1-3 mutant and CAD1 backgrounds. Six lines, with all possible combinations of hma2, hma4 and cad1 mutations, were constructed. The lines were tested for Cd-sensitivity on agar medium, and radioactive (109)Cd was used to measure Cd uptake and translocation from root to shoot over periods of up to 6 d. In hma4 and hma2,hma4, but not hma2, root-to-shoot Cd translocation was decreased to about 60 and 2%, respectively, of that in the wild-type. Cd sensitivity increased approximately twofold in the hma2,hma4 mutant in both CAD1 and cad1 backgrounds. PC deficiency resulted in an increase in shoot Cd concentrations. The near-complete abolition of root-to-shoot Cd translocation resulting from the loss of function of HMA2 and HMA4 demonstrates they are the major mechanism for Cd translocation in Arabidopsis thaliana |
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| Beschreibung: | Date Completed 06.02.2009 Date Revised 30.09.2020 published: Print CommentIn: New Phytol. 2009;181(1):3-5. - PMID 19076712 Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/j.1469-8137.2008.02638.x |