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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1021/la8033098
|2 doi
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|a pubmed24n0616.xml
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|a (NLM)19049394
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|a DE-627
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|e rakwb
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|a eng
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|a Reed, Jason
|e verfasserin
|4 aut
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| 245 |
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|a In situ mechanical interferometry of matrigel films
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|c 2009
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 27.02.2009
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|a Date Revised 11.03.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a Many biological materials and cell substrates are very soft (Young's modulus <500 Pa) and it is difficult to characterize their mechanical properties. Here we report local elasticity of the surface layers of Matrigel films used for cell culture. We used a new measurement technology, mechanical imaging interferometry, to obtain point mechanical measurements over micron-sized areas. The median values of 650 +/- 400 Pa (# measurements, n = 50), determined by the Hertz contact model, agree well with bulk measurements; however, on the microscale, the films were heterogeneous and contained regions distinctly stiffer than average (1-2 kPa). The first measurement of yield strengths of 170 +/- 100 Pa (n = 43) indicates that Matrigel films deform plastically at stress levels of similar scale to cell tractional forces
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Drug Combinations
|2 NLM
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|a Laminin
|2 NLM
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|a Proteoglycans
|2 NLM
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|a matrigel
|2 NLM
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|a 119978-18-6
|2 NLM
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|a Collagen
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|a 9007-34-5
|2 NLM
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|a Walczak, Wanda J
|e verfasserin
|4 aut
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|a Petzold, Odessa N
|e verfasserin
|4 aut
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|a Gimzewski, James K
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 25(2009), 1 vom: 06. Jan., Seite 36-9
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:25
|g year:2009
|g number:1
|g day:06
|g month:01
|g pages:36-9
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|u http://dx.doi.org/10.1021/la8033098
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