Evidence for leaflet-dependent redistribution of charged molecules in fluid supported phospholipid bilayers

The asymmetric distribution of charged molecules between the leaflets of solid-substrate-supported phospholipid bilayers is studied using imaging ellipsometry, fluorescence microscopy, and numerical solutions of the Poisson-Boltzmann equation. Experiments are facilitated by the use of patterned subs...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 24(2008), 23 vom: 02. Dez., Seite 13250-3
1. Verfasser: Shreve, Andrew P (VerfasserIn)
Weitere Verfasser: Howland, Michael C, Sapuri-Butti, Annapoorna R, Allen, Toby W, Parikh, Atul N
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Lipid Bilayers Membranes, Artificial Proteolipids Silicon Dioxide 7631-86-9
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520 |a The asymmetric distribution of charged molecules between the leaflets of solid-substrate-supported phospholipid bilayers is studied using imaging ellipsometry, fluorescence microscopy, and numerical solutions of the Poisson-Boltzmann equation. Experiments are facilitated by the use of patterned substrates that allow for side-by-side comparison of lipid monolayers and supported bilayers. On silica surfaces, negatively charged lipid components are shown to be enriched in the outer leaflet of a supported bilayer system at modest salt concentrations. The approaches developed provide a general means for determining asymmetries of charged components in supported lipid bilayers 
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650 4 |a Research Support, N.I.H., Extramural 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
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700 1 |a Howland, Michael C  |e verfasserin  |4 aut 
700 1 |a Sapuri-Butti, Annapoorna R  |e verfasserin  |4 aut 
700 1 |a Allen, Toby W  |e verfasserin  |4 aut 
700 1 |a Parikh, Atul N  |e verfasserin  |4 aut 
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