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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1021/la802097z
|2 doi
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|a pubmed24n0615.xml
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|a eng
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| 100 |
1 |
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|a Sun, Y S
|e verfasserin
|4 aut
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|a Effect of fluorescently labeling protein probes on kinetics of protein-ligand reactions
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 23.03.2009
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|a Date Revised 13.11.2018
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|a published: Print
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|a Citation Status MEDLINE
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|a We studied the effect of fluorescently labeling proteins on protein-ligand reactions. Unlabeled ligands (streptavidin-binding peptides and rabbit immunoglobulin G (IgG) as antigen targets) are immobilized on epoxy-functionalized glass slides. Unlabeled and Cy3-labeled protein probes from the same batch (streptavidin and goat antibodies) subsequently react with the surface-immobilized targets. By monitoring in situ the surface mass density change using an oblique-incidence reflectivity difference scanning microscope (a label-free detector), we measured k(on) and k(off) for streptavidin-peptide reactions and antibody-antigen reaction. We found that (1) equilibrium dissociation constants, defined as K(D) = k(off)/k(on), for streptavidin-peptide reactions increases by a factor of 3-4 when the solution-phase streptavidin is labeled with Cy3 dye and (2) K(D) for reactions of solution-phase goat anti-rabbit antibodies with rabbit IgG targets also change significantly when the goat antibodies are labeled with Cy3 dye
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Carbocyanines
|2 NLM
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|a Epoxy Compounds
|2 NLM
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|a Immunoglobulin G
|2 NLM
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|a Ligands
|2 NLM
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|a Oligopeptides
|2 NLM
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|a cyanine dye 3
|2 NLM
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|a Streptavidin
|2 NLM
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| 650 |
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|a 9013-20-1
|2 NLM
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| 700 |
1 |
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|a Landry, J P
|e verfasserin
|4 aut
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1 |
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|a Fei, Y Y
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhu, X D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Luo, J T
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, X B
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lam, K S
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 24(2008), 23 vom: 02. Dez., Seite 13399-405
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:24
|g year:2008
|g number:23
|g day:02
|g month:12
|g pages:13399-405
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|u http://dx.doi.org/10.1021/la802097z
|3 Volltext
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